Ultrasensitive quantitative detection of small molecules with rapid lateral-flow assay based on high-affinity bifunctional ligand and magnetic nanolabels
| Autor: | N. V. Guteneva, Petr I. Nikitin, Maxim P. Nikitin, A. V. Orlov, A.V. Pushkarev, E.N. Mochalova, A.V. Lunin, S.L. Znoyko |
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| Rok vydání: | 2018 |
| Předmět: |
Biotin
02 engineering and technology Ligands 01 natural sciences Biochemistry Chromatography Affinity Analytical Chemistry Small Molecule Libraries chemistry.chemical_compound Humans Environmental Chemistry Molecule Magnetite Nanoparticles Bifunctional Spectroscopy Detection limit Chemistry 010401 analytical chemistry Antibodies Monoclonal 021001 nanoscience & nanotechnology Ligand (biochemistry) Small molecule Combinatorial chemistry 0104 chemical sciences Highly sensitive Thyroxine Magnetic nanoparticles 0210 nano-technology Biosensor |
| Zdroj: | Analytica Chimica Acta. 1034:161-167 |
| ISSN: | 0003-2670 |
| Popis: | An ultrasensitive lateral-flow assay is developed for rapid quantitative detection of small molecules on-site. The conceptual novelty, which transfers lateral-flow assays to the category of highly sensitive quantitative systems, is due to employment of a bifunctional ligand combined with volumetric registration of magnetic nanolabels. The ligand provides extremely high affinity for trapping the nanolabels and, simultaneously, efficiently competes with the analyzed molecules for the limited quantity of antigen-binding sites on the nanolabels. The developed assay has been demonstrated as the first express method for measuring in human serum of free thyroxine (fT4). The limit of detection is 20 fМ or 16 fg/ml at the assay time30 min with the dynamic range of 3 orders. Besides, we present the results of first characterization of kinetic parameters of interaction between free thyroxine and monoclonal antibody, as well as of competitive relationship between fT4 and fT4-biotin. The proposed universal platform can be used for ultrasensitive detection of small molecules in human in vitro diagnostics, veterinary, biosafety and counter-terrorism, food quality control, environmental monitoring, etc., as well as for search of new, previously undetectable, diagnostic markers in medicine. |
| Databáze: | OpenAIRE |
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