Bono1: a gene associated with regions of deposition of bone and dentine
| Autor: | Irma Thesleff, Elina Järvinen, Martyn J. James |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Bone sialoprotein
Sialoglycoproteins Gene Expression Biology Bone and Bones 03 medical and health sciences Mice 0302 clinical medicine stomatognathic system Dentin sialophosphoprotein Genetics medicine Animals Integrin-Binding Sialoprotein Protein Precursors Molecular Biology Endochondral ossification In Situ Hybridization 030304 developmental biology DNA Primers Expressed Sequence Tags 0303 health sciences Extracellular Matrix Proteins Odontoblasts Ossification Reverse Transcriptase Polymerase Chain Reaction Skull 030206 dentistry Anatomy Phosphoproteins Embryonic stem cell Cell biology Insulin-Like Growth Factor Binding Proteins stomatognathic diseases Odontoblast medicine.anatomical_structure Intramembranous ossification biology.protein medicine.symptom Developmental Biology |
| Zdroj: | Gene expression patterns : GEP. 4(5) |
| ISSN: | 1567-133X |
| Popis: | We have examined the mRNA expression pattern of the murine expressed sequence tag (EST) clone AI842353 in embryonic and early postnatal mice. Expression was strongly and specifically localised to developing bones and odontoblasts in teeth, therefore we have named this gene Bono1 (Bone and odontoblasts). Bono1, which has human, rat and chicken orthologues designated as FKSG28 was expressed in most ossification regions of the head including calvarial bones, skull and jaws. Expression was localised to osteoblasts derived from both intramembraneous and endochondral ossification processes. Comparative analysis of the expression of Bono1 in the mandible with Bone sialoprotein (BSP), a marker of advanced osteoblastogenesis, revealed that Bono1 expression starts later in the osteoblast cell lineage than BSP. In the tooth, Bono1 was localised in secretory odontoblasts. This expression was complementary to BSP, which was only present in early pre-odontoblasts. In secretory odontoblasts, Bono1 was shown to be co-expressed with Dentin sialophosphoprotein (DSPP). In summary, Bono1 was expressed in functional osteoblasts and odontoblasts and was associated with regions of matrix mineralization. |
| Databáze: | OpenAIRE |
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