Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes
| Autor: | Régine Ilboudo, Sessi Frida Tovo, D. Ouermi, Albert Théophane Yonli, Rogomenoma Alice Ouedraogo, Florencia Wendkuuni Djigma, Jacques Simpore, Lassana Sangaré, Abdoul Karim Ouattara, Pegdwendé Abel Sorgho, Amana Metuor Dabire, Essi Etonam Dovo, Théodora Mahoukèdè Zohoncon, Charlemagne Ouedraogo, Rahimatou Yasmine Tiemtoré, Dorcas Obiri-Yeboah |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
HPV
Drug resistance Biology medicine.disease_cause Neisseriae gonorrhoeae STIs Article Antibiotic resistance medicine Sex organ sex workers General Immunology and Microbiology Molecular epidemiology resistance genes Public Health Environmental and Occupational Health medicine.disease biology.organism_classification Virology female genital diseases and pregnancy complications Infectious Diseases PCR Coinfection Neisseria gonorrhoeae Medicine Chlamydia trachomatis Mycoplasma genitalium |
| Zdroj: | Tropical Medicine and Infectious Disease, Vol 6, Iss 90, p 90 (2021) Tropical Medicine and Infectious Disease Volume 6 Issue 2 |
| ISSN: | 2414-6366 |
| Popis: | Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit “HPV Genotypes 14 Real-TM Quant” from SACACE Biotechnologies®, detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the real-time multiplex amplification kit N. gonorrhoeae/C. trachomatis/M. genitalium/T. vaginalis Real-TM, allowing their simultaneous detection, was used. The women were aged 17–50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), Neisseria gonorrhoeae (13.74%), Chlamydia trachomatis (11.54%) and Mycoplasma genitalium (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were bla QNR B 24.00%, bla GES 22.00%, bla SHV 17.00%, blaCTX-M 13.00% and bla QNR S 1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers of West and Central African origin working in Ouagadougou. |
| Databáze: | OpenAIRE |
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