Molecular Characterization of Imipenem-ResistantPseudomonas aeruginosain Hiroshima, Japan
| Autor: | Hideyuki Itaha, Tuguru Usui, Naomasa Gotoh, Shuntaro Kouda, Seiya Kashiyama, Shizue Hayashida, Hitoshi Komatsuzawa, Masao Kuwabara, Motoyuki Sugai, Megumi Sasaki, Tadahiro Kohara, Manami Kadono, Masaru Ohara, Yoshihiro Fujiue, Takashi Yokoyama, Makoto Onodera |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Male
Imipenem Immunology Drug resistance Biology medicine.disease_cause Microbiology beta-Lactam Resistance beta-Lactamases law.invention Japan law Levofloxacin Drug Resistance Multiple Bacterial Virology Pulsed-field gel electrophoresis medicine Humans Pseudomonas Infections Phylogeny Polymerase chain reaction Aged Aged 80 and over Cross Infection Molecular epidemiology Pseudomonas aeruginosa biochemical phenomena metabolism and nutrition bacterial infections and mycoses Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Multigene Family Female Efflux medicine.drug |
| Zdroj: | Microbiology and Immunology. 51:271-277 |
| ISSN: | 0385-5600 |
| DOI: | 10.1111/j.1348-0421.2007.tb03908.x |
| Popis: | Pseudomonas aeruginosa showing resistance to imipenem were found in 100 of 1,058 strains (9.5%) from six hospitals (a-f) in Hiroshima City, Japan. Of the 100 strains, 14 (14%) were double disk synergy test positive using sodium mercaptoacetic acid disks, and 18 (18%) were bla(IMP-1) or bla(VIM-2) allele positive by polymerase chain reaction (PCR). Among 100 imipenem-resistant strains, 32 were categorized into multi-drug resistant strains, in which 13 were positive for the metallo-beta-lactamase gene. Fifty-one strains (51%) among the 100 imipenem-resistant strains had elevated RND efflux pump activity against levofloxacin. But only 6 of 51 strains were classified as multi-drug resistant strains. The pulsed field gel electrophoresis analysis of the Spe I-digested DNA from the 100 isolates suggested not only clonal spread but spread of heterogeneous clones started to contribute to the prevalence of metallo-beta-lactamase producing P. aeruginosa strains in Japanese hospitals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text