Effect of papaverine on endothelial cell harvest from canine external jugular veins
| Autor: | Reitz-Vick Dm, Bendick Pj, Fietsam R, Timothy Ranval, Laurace E. Townsend, John L. Glover |
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| Rok vydání: | 1991 |
| Předmět: |
Pathology
medicine.medical_specialty Physiology Cell Survival Cytological Techniques Cell Count Biology Dogs Culture Techniques Papaverine medicine Carnivora Animals Vascular disease Fissipedia medicine.disease biology.organism_classification Peripheral Culture Media Endothelial stem cell Circulatory system Endothelium Vascular Jugular Veins Cardiology and Cardiovascular Medicine External jugular vein medicine.drug |
| Zdroj: | Blood vessels. 28(6) |
| ISSN: | 0303-6847 |
| Popis: | Endothelial cell seeding procedures have been developed to line prosthetic bypass grafts used in peripheral vascular disease; however, because of current inefficient cell harvest techniques a high ratio of vein-to-graft area is necessary. This study was done to determine if the use of papaverine, a smooth muscle cell relaxant, would affect the number or viability of endothelial cells harvested from canine external jugular veins. Using a 0.12 mg/ml solution of papaverine in tissue culture medium to bathe the veins during disscetion and excision, the viable cell yield was 2.20 ± 1.16 (cells × 104/cm2). A control group of veins using standard dissection technique gave a yield of 0.97 ± 0.40 (p = 0.025). A second group of veins dissected while bathed in tissue culture medium alone gave a yield of 1.82 ± 0.75, compared to a yield of 2.73 ± 0.45 for papaverine harvested veins (p = 0.009). Percent cell viability was not significantly different for any of the groups: 73, 70, and 76% for papaverine, control and media only veins, respectively. The papaverine-harvested cells and those harvested with medium alone grew to 95% confluence in tissue culture in 9.8 ± 1.1 and 9.9 ± 0.9 days, respectively. Compared to conventional surgical techniques, use of papaverine more than doubled the endothelial cell yield from excised vein segments without adversely affecting viability or rate of growth in cell culture. |
| Databáze: | OpenAIRE |
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