Biofilm-forming capacity of blood-borne Candida albicans strains and effects of antifungal agents
| Autor: | Müge Demirbilek, Hanni Turan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Antifungal Agents Itraconazole 030106 microbiology Microbial Sensitivity Tests Microbiology MBIC 03 medical and health sciences chemistry.chemical_compound Echinocandins Amphotericin B Candida albicans medicine Inhibition of biofilm formation Humans Calgary method Candida biology Biofilm General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Corpus albicans 030104 developmental biology Christensen method chemistry Biofilms Anidulafungin Caspofungin Fluconazole medicine.drug |
| Popis: | Infections related to Candida albicans biofilms and subsequent antifungal resistance have become more common with the increased use of indwelling medical devices. Regimens for preventing fungal biofilm formation are needed, particularly in high-risk patients. In this study, we investigated the biofilm formation rate of multiple strains of Candida albicans (n = 162 clinical isolates), their antifungal susceptibility patterns, and the efficacy of certain antifungals for preventing biofilm formation. Biofilm formation was graded using a modified Christensen's 96-well plate method. We further analyzed 30 randomly chosen intense biofilm-forming isolates using the XTT method. Minimum biofilm inhibition concentrations (MBIC) of caspofungin, micafungin, anidulafungin, fluconazole, voriconazole, posaconazole, itraconazole, and amphotericin B were determined using the modified Calgary biofilm method. In addition, the inhibitory effects of antifungal agents on biofilm formation were investigated. Our study showed weak, moderate, and extensive biofilm formation in 29% (n = 47), 38% (n = 61), and 23% (n = 37) of the isolates, respectively. We found that echinocandins had the lowest MBIC values and that itraconazole inhibited biofilm formation in more isolates (26/32; 81.3%) than other tested agents. In conclusion, echinocandins were most effective against formed biofilms, while itraconazole was most effective for preventing biofilm formation. Standardized methods are needed for biofilm antifungal sensitivity tests when determining the treatment and prophylaxis of C. albicans infections. (c) 2017 Asociacion Argentina de Microbiologia. Published by Elsevier Espana, S.L.U. |
| Databáze: | OpenAIRE |
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