Differential gene-expression and host-response profiles against avian influenza virus within the chicken lung due to anatomy and airflow
| Autor: | Daphne A. van Haarlem, Sylvia S. Reemers, Marian J. A. Groot Koerkamp, Lonneke Vervelde |
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| Rok vydání: | 2009 |
| Předmět: |
Orthomyxoviridae
Respiratory chain Gene Expression medicine.disease_cause Virus Virology Gene expression Influenza A Virus H9N2 Subtype medicine Influenza A virus Animals Lung biology Anatomy respiratory system biology.organism_classification Specific Pathogen-Free Organisms Trachea medicine.anatomical_structure Influenza in Birds Host-Pathogen Interactions Respiratory virus Chickens Respiratory tract |
| Zdroj: | Journal of General Virology. 90:2134-2146 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Sampling the complete organ instead of defined parts might affect analysis at both the cellular and transcriptional levels. We defined host responses to H9N2 avian influenza virus (AIV) in trachea and different parts of the lung. Chickens were spray-inoculated with either saline or H9N2 AIV. Trachea and lung were sampled at 1 and 3 days post-inoculation (p.i.) for immunocytochemistry, real-time quantitative RT-PCR and gene-expression profiling. The trachea was divided into upper and lower parts and the lung into four segments, according to anatomy and airflow. Two segments contained the primary and secondary bronchi, cranial versus caudal (parts L1 and L3), and two segments contained the tertiary bronchi, cranial versus caudal (parts L2 and L4). Between the upper and lower trachea in both control and infected birds, minor differences in gene expression and host responses were found. In the lung of control birds, differences in anatomy were reflected in gene expression, and in the lung of infected birds, virus deposition enhanced the differences in gene expression. Differential gene expression in trachea and lung suggested common responses to a wide range of agents and site-specific responses. In trachea, site-specific responses were related to heat shock and lysozyme activity. In lung L1, which contained most virus, site-specific responses were related to genes involved in innate responses, interleukin activity and endocytosis. Our study indicates that the anatomy of the chicken lung must be taken into account when investigating in vivo responses to respiratory virus infections. |
| Databáze: | OpenAIRE |
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