Burrowing is a sensitive behavioural assay for monitoring general wellbeing during dextran sulfate sodium colitis in laboratory mice
| Autor: | Katharina Leucht, Nikola Cesarovic, Flora Nicholls, Gerhard Rogler, Margarete Arras, Michaela Caj, Martin Hausmann, Paulin Jirkof |
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| Přispěvatelé: | University of Zurich, Arras, M |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
3400 General Veterinary Pain 610 Medicine & health Spleen Inflammation Animal Welfare Inflammatory bowel disease Gastroenterology Pathogenesis Mice Stress Physiological Internal medicine medicine Animals Colitis Acute colitis General Veterinary Behavior Animal Adrenal gland business.industry Histology 11359 Institute for Regenerative Medicine (IREM) Colonoscopy medicine.disease Mice Inbred C57BL Disease Models Animal 10022 Division of Surgical Research 10219 Clinic for Gastroenterology and Hepatology medicine.anatomical_structure Immunology 570 Life sciences biology Animal Science and Zoology 1103 Animal Science and Zoology medicine.symptom business |
| Zdroj: | Laboratory animals. 47(4) |
| ISSN: | 1758-1117 |
| Popis: | An impaired intestinal epithelial barrier is thought to be a major factor in the pathogenesis of human inflammatory bowel disease (IBD). IBD is frequently investigated by inducing a damaged barrier in murine models of colitis. This can be done by feeding mice with dextran sulfate sodium (DSS) polymers in their drinking water. Refinement measures should focus on alleviating unnecessary suffering during this probably painful condition. Appropriate parameters are needed to decide when to terminate the experiments. Our aim was to investigate whether a change in burrowing behaviour is a sensitive measure of animal welfare in murine models of colitis. Acute colitis was induced in C57BL/6 mice with 2.0% DSS over nine days. The burrowing test is based on the species-typical behaviour of mice to spontaneously displace items from tubes within their home cage. As a burrowing apparatus, a water bottle (250 mL, 150 mm length, 55 mm diameter) filled with 138–142 g of pellets of the animal’s diet was used. The presence of intestinal inflammation as a result of acute DSS-induced colitis was confirmed by a decrease in body weight, colon length and an increase of murine endoscopic index of colitis severity, histological score and spleen weight in the group receiving DSS as compared with the control group. An onset of intestinal inflammation correlated with a significant decrease in burrowing behaviour ( P |
| Databáze: | OpenAIRE |
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