The effect of perinatal TCDD exposure on caries susceptibility in rats
| Autor: | Juha Tuukkanen, Matti Viluksela, Hanna M. Miettinen, Satu Alaluusua, Rita Sorvari, Mari Murtomaa |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Polychlorinated Dibenzodioxins Offspring Physiology Dentistry Dental Caries Toxicology Diet Cariogenic Dental Caries Susceptibility chemistry.chemical_compound stomatognathic system Pregnancy medicine Weaning Animals heterocyclic compounds Dental Enamel Minerals biology Enamel paint business.industry Abnormalities Drug-Induced Rats Inbred Strains biology.organism_classification Tooth enamel Streptococcus mutans Molar Rats stomatognathic diseases medicine.anatomical_structure chemistry visual_art Prenatal Exposure Delayed Effects Dentin visual_art.visual_art_medium Gestation Environmental Pollutants Female Disease Susceptibility business Toxicant |
| Zdroj: | Toxicological sciences : an official journal of the Society of Toxicology. 91(2) |
| ISSN: | 1096-6080 |
| Popis: | 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), the model compound of polychlorinated dibenzo-p-dioxins and furans, is a potent toxicant with the ability to hamper development. Accidental exposure to TCDD has been linked with various developmental dental aberrations in humans, and experimentally it has been shown that TCDD causes, among other defects, hypomineralization of dental hard tissues in rodents. Here, we studied the effect of very low perinatal TCDD exposure on dental caries susceptibility and mineral composition of tooth enamel in rats. Pregnant line C rats (rat line developed in our laboratory) were dosed 0.03-1.0 microg/kg TCDD on gestation day 15 and allowed to give birth and nurse until weaning on postnatal day 21. The offspring were challenged with cariogenic treatment including sugar-rich diet and three inoculations with Streptococcus mutans. Control groups involved animals with or without cariogenic challenge or TCDD treatment. The number of caries lesions in left lower molars was determined by Schiff's staining after 8 weeks of weaning. TCDD treatment increased cariogenic lesions in the enamel at the lowest maternal dose used, 0.03 microg/kg, and at the highest maternal dose, 1 microg/kg, the lesions extended through the enamel to dentin more frequently. Changes in mineral composition measured by electron probe microanalyzer, scanning electron microscopy, and energy-dispersive spectrometry could not explain the increased caries susceptibility. In conclusion, perinatal TCDD exposure can render rat molars more susceptible to caries. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|