Nitrate transport capacity of the Arabidopsis thaliana NRT2 family members and their interactions with AtNAR2.1
| Autor: | Zorica Kotur, Nenah Mackenzie, Stephen D. Tyerman, Brent N. Kaiser, Anthony D. M. Glass, Sunita A. Ramesh |
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| Rok vydání: | 2012 |
| Předmět: |
Physiology
Recombinant Fusion Proteins Xenopus Nitrogen assimilation Anion Transport Proteins Arabidopsis Plant Science Plant Roots RNA Complementary chemistry.chemical_compound Bimolecular fluorescence complementation Nitrate Two-Hybrid System Techniques Protein Interaction Mapping Animals Arabidopsis thaliana Nitrates Nitrogen Isotopes biology Arabidopsis Proteins Protoplasts Biological Transport biology.organism_classification Yeast Plant Leaves chemistry Biochemistry RNA Plant Nitrate transport Mutation Oocytes |
| Zdroj: | New Phytologist. 194:724-731 |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/j.1469-8137.2012.04094.x |
| Popis: | • Interactions between the Arabidopsis NitRate Transporter (AtNRT2.1) and Nitrate Assimilation Related protein (AtNAR2.1, also known as AtNRT3.1) have been well documented, and confirmed by the demonstration that AtNRT2.1 and AtNAR2.1 form a 150-kDa plasma membrane complex, thought to constitute the high-affinity nitrate transporter of Arabidopsis thaliana roots. Here, we have investigated interactions between the remaining AtNRT2 family members (AtNRT2.2 to AtNRT2.7) and AtNAR2.1, and their capacity for nitrate transport. • Three different systems were used to examine possible interactions with AtNAR2.1: membrane yeast split-ubiquitin, bimolecular fluorescence complementation in A. thaliana protoplasts and nitrate uptake in Xenopus oocytes. • All NRT2s, except for AtNRT2.7, restored growth and β-galactosidase activity in the yeast split-ubiquitin system, and split-YFP fluorescence in A. thaliana protoplasts only when co-expressed with AtNAR2.1. Thus, except for AtNRT2.7, all other NRT2 transporters interact strongly with AtNAR2.1. • Co-injection into Xenopus oocytes of cRNA of all NRT2 genes together with cRNA of AtNAR2.1 resulted in statistically significant increases of uptake over and above that resulting from single cRNA injections. |
| Databáze: | OpenAIRE |
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