Soy-tomato enriched diet reduces inflammation and disease severity in a pre-clinical model of chronic pancreatitis
| Autor: | Molly Torok, Jessica L. Cooperstone, Alice Hinton, Jenna L. Miller, Darwin L. Conwell, Rahul Kumar, Phil A. Hart, Fouad Choueiry, Debasmita Mukherjee, Samuel K. Kulp, Thomas A. Mace, Anna Deems, William E. Carson, Mallory J. DiVincenzo, Jose Angel Maranon, Connor Geraghty, Christopher C. Coss |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Necrosis Science medicine.medical_treatment Spleen Inflammation Severity of Illness Index Article Mice 03 medical and health sciences 0302 clinical medicine Immune system Solanum lycopersicum Fibrosis Pancreatitis Chronic Internal medicine medicine Animals Humans Multidisciplinary business.industry medicine.disease Bioavailability Disease Models Animal 030104 developmental biology medicine.anatomical_structure Cytokine Endocrinology Pancreatitis Fruit 030220 oncology & carcinogenesis Medicine Soybeans medicine.symptom business |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-14 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Chronic pancreatitis (CP) is a fibro-inflammatory syndrome in individuals who develop persistent pathological responses to parenchymal injury or stress. Novel therapeutic or dietary interventions that could lessen inflammation in this disease could significantly improve quality of life in patients with CP. Complex dietary foods like soy and tomatoes are composed of active metabolites with anti-inflammatory effects. Data from our group reports that bioactive agents in soy and tomatoes can reduce pro-inflammatory cytokines and suppressive immune populations. Additionally, our team has developed a novel soy-tomato juice currently being studied in healthy individuals with no toxicities, and good compliance and bioavailability. Thus, we hypothesize that administration of a soy-tomato enriched diet can reduce inflammation and severity of CP. C57BL/6 mice were injected intraperitoneally with 50 μg/kg caeurlein (7 hourly injections, twice weekly) for 6 weeks to induce CP. After 4 weeks of caerulein injections, mice were administered a control or a soy-tomato enriched diet for 2 weeks. Disease severity was measured via immunohistochemical analysis of pancreata measuring loss of acini, fibrosis, inflammation, and necrosis. Serum lipase and amylase levels were analyzed at the end of the study. Inflammatory factors in the serum and pancreas, and immune populations in the spleen of mice were analyzed by cytokine multiplex detection, qRT-PCR, and flow cytometry respectively. Infra-red (IR) sensing of mice was used to monitor spontaneous activity and distress of mice. Mice fed a soy-tomato enriched diet had a significantly reduced level of inflammation and severity of CP (p = 0.032) compared to mice administered a control diet with restored serum lipase and amylase levels (p p p = 0.055) and CP mice on a control diet were determined to spend more time at rest (p = 0.053). These pre-clinical results indicate that a soy-tomato enriched diet may be a novel treatment approach to reduce inflammation and pain in patients with CP. |
| Databáze: | OpenAIRE |
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