Development of an HPLC-DAD method for the determination of five alkaloids in Stephania yunnanensis Lo and in rat plasma after oral dose of Stephania yunnanensis Lo extracts
| Autor: | Duolong Di, Yaming Zhang, Yanxia Zhang, Jun-Xi Liu, Aiyi Xin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Clinical Biochemistry Administration Oral Tetrahydropalmatine 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Alkaloids Oral administration Limit of Detection Drug Discovery Animals heterocyclic compounds Aporphine Isoquinoline Stephania Molecular Biology Sinomenine Chromatography High Pressure Liquid Pharmacology Chromatography biology Plant Extracts 010401 analytical chemistry Reproducibility of Results Palmatine General Medicine biology.organism_classification 0104 chemical sciences Rats 030104 developmental biology chemistry Linear Models Female Stephania yunnanensis |
| Zdroj: | Biomedical chromatography : BMC. 32(10) |
| ISSN: | 1099-0801 |
| Popis: | For the rational utilization and the quantitative quality control of the Stephania yunnanensis Lo, an HPLC-DAD method was developed for the quantitative and simultaneous determination of five alkaloids in rat plasma (stepharine, sinomenine, palmatine, isocorydine and tetrahydropalmatine), which were the main active chemical constituents of this plant and belong to four kinds of isoquinoline-type alkaloids (protoberberine, morphine, aporphine and protaporphine alkaloids). The contents of five alkaloids ranged from 0.09 to 2.32% (w/w). The method validation was tested for the linearity (r2 > 0.9975), precision (intra-day RSD |
| Databáze: | OpenAIRE |
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