Identification of TRAK1 (Trafficking protein, kinesin-binding 1) as MGb2-Ag: a novel cancer biomarker
| Autor: | Gui Ren, Bin Jin, Yongzhan Nie, Zhenxiong Liu, Daiming Fan, Xiong Chen, Faming Zhang, Jun Wang, Kai Li, Yuanyuan Lu, Xin Wang |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty Blotting Western Epitope Antibodies Antigen Stomach Neoplasms Cell Line Tumor medicine Biomarkers Tumor Humans Immunoprecipitation Kinesin binding biology Chemistry Cancer Middle Aged medicine.disease Molecular biology Immunohistochemistry digestive system diseases Adaptor Proteins Vesicular Transport Oncology Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Cancer cell biology.protein Adenocarcinoma Electrophoresis Polyacrylamide Gel Female Antibody Immunostaining |
| Zdroj: | Cancer letters. 274(2) |
| ISSN: | 1872-7980 |
| Popis: | The present study aimed to describe the characterization of an antibody MGb2 that reacts with an epitope on gastric cancer cells, and identification of MGb2 antigen (MGb2-Ag). Immunostaining revealed its distribution in human tissues and demonstrated that the positive rate of MGb2-Ag was 81.48% in gastric cancer, 100% in gastric signet-ring cell carcinoma and mucinous adenocarcinoma, 13.16% in precancerous conditions, and 0% in chronic superficial gastritis. Using Western blotting, immunoprecipitation and MALDI-TOF MS (matrix assisted laser desorption/ionization time-of-flight mass spectrometry), MGb2-Ag was identified as TRAK1 (Trafficking protein, kinesin-binding 1), a new molecular gained limited recognition. Both MGb2 and commercial anti-TRAK1 Ab recognized prokaryotic expressed TRAK1. Immunostaining characteristics of TRAK1 were identical with MGb2-Ag in continuous sections of paraffin-embedded tissues of gastric tissues. This is the first report that TRAK1/MGb2-Ag is a promising diagnostic marker for gastric cancer and may help to detect signet-ring cell carcinoma and mucinous adenocarcinoma. |
| Databáze: | OpenAIRE |
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