Signal pathways, diseases, and functions associated with the miR-19a/92a cluster and the use of berberine to modulate the expression of this cluster in multiple myeloma cells
| Autor: | Zhao Yin, Rong Ning, Chunmin Gu, Maoxiao Feng, Juhua Yang, Yumin Li, Jia Fei, Yanjun Liu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Berberine Cell Survival Health Toxicology and Mutagenesis Down-Regulation Biology Real-Time Polymerase Chain Reaction Toxicology Disease cluster Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Movement Cell Line Tumor microRNA medicine Humans Viability assay Molecular Biology Multiple myeloma Cell Proliferation Signal Pathways Cell growth General Medicine medicine.disease Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology chemistry Multigene Family 030220 oncology & carcinogenesis Cancer research Molecular Medicine Gene-Environment Interaction Signal transduction Multiple Myeloma Signal Transduction |
| Zdroj: | Journal of Biochemical and Molecular Toxicology. 32:e22057 |
| ISSN: | 1095-6670 |
| Popis: | Background Berberine downregulated miR-19a/92a cluster expression in multiple myeloma (MM) cells. Methods The cell viability of MM cells after berberine treatment was measured by CCK8 assay. qRT-PCR assay validated miR-19a/92a expression in multiple myeloma cells. TAM database analyzed miR-19a/92a-associated disease. miREnvironment database revealed that effects of environmental factors on the miR-19a/92a cluster. By targeting the seed region in the miRNA, the role of t-anti-miR-19a/92a cluster was evaluated by cell proliferation, migration, and colony formation. Results Berberine inhibited the cell viability of MM cells and downregulated the expression of miR-19a/92a. Seven kinds of hematological malignancies are closely associated with miR-19a/92a expression. By targeting the seed region of the miRNA, t-anti-miR-19a/92a significantly inhibits multiple myeloma cell proliferation, migration, and colony formation. Conclusion Our findings may exhibit that miR-19a/92a cluster is a therapeutic target for MM and provide new mechanistic insight into the anti-MM effects of certain compounds in traditional Chinese herbal medicines. |
| Databáze: | OpenAIRE |
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