Paving the way to single-molecule protein sequencing
| Autor: | Cees Dekker, Laura Restrepo-Pérez, Chirlmin Joo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Proteomics
0301 basic medicine Computer science Biomedical Engineering Bioengineering Computational biology Condensed Matter Physics Atomic and Molecular Physics and Optics Protein content Nanopores 03 medical and health sciences 030104 developmental biology Protein sequencing Sequence Analysis Protein Humans General Materials Science Electrical and Electronic Engineering |
| Zdroj: | Nature Nanotechnology |
| ISSN: | 1748-3395 1748-3387 |
| Popis: | Proteins are major building blocks of life. The protein content of a cell and an organism provides key information for the understanding of biological processes and disease. Despite the importance of protein analysis, only a handful of techniques are available to determine protein sequences, and these methods face limitations, for example, requiring a sizable amount of sample. Single-molecule techniques would revolutionize proteomics research, providing ultimate sensitivity for the detection of low-abundance proteins and the realization of single-cell proteomics. In recent years, novel single-molecule protein sequencing schemes that use fluorescence, tunnelling currents and nanopores have been proposed. Here, we present a review of these approaches, together with the first experimental efforts towards their realization. We discuss their advantages and drawbacks, and present our perspective on the development of single-molecule protein sequencing techniques. |
| Databáze: | OpenAIRE |
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