Proteomic pattern of implantative human endometrial fluid in in vitro fertilization cycles
| Autor: | Daniel Nagore, Felix Elortza, Sara Quevedo, Marcos Ferrando, Rosario Mendoza, Begoña Prieto, Roberto Matorras, Antonia Expósito, Maria Diaz-Nuñez, Blanca Corral, Aintzane Rabanal, Amagoia Ametzazurra |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Proteomics Pregnancy Rate medicine.medical_treatment Population Fertilization in Vitro Endometrium Mass Spectrometry Andrology 03 medical and health sciences 0302 clinical medicine Pregnancy Medicine Humans Embryo Implantation education Annexin A2 CapZ Actin Capping Protein education.field_of_study 030219 obstetrics & reproductive medicine Two-dimensional gel electrophoresis In vitro fertilisation business.industry Obstetrics and Gynecology General Medicine medicine.disease Embryo Transfer Embryo transfer Pregnancy rate 030104 developmental biology medicine.anatomical_structure Stathmin Female business |
| Zdroj: | Archives of gynecology and obstetrics. 297(6) |
| ISSN: | 1432-0711 |
| Popis: | To assess whether there are proteins in endometrial fluid aspirate (EFA) that predict implantation. The population under study consisted of 285 women undergoing embryo transfer (ET). Endometrial fluid aspiration was performed immediately before ET. Results of proteomic analysis of EFA were compared between 33 cases who achieved pregnancy and 33 who did not. Samples were analysed by 2D electrophoresis and mass spectrometry. Blood samples were studied by ELISA Pregnancy rates and maternal complications were compared to those in women refusing aspiration. We found 23 proteins differentially expressed in the EFA in conception cycles: 4 up-regulated proteins and 19 down-regulated (FC = 0.31 0.78) (among others, arginase-1, actin B, PARK-7, cofilin-1, stathmin, annexin-2 and CAPZB). Among the five studied proteins that were differentially expressed in EFA, none was differentially expressed in serum. The aspiration procedure had no impact on pregnancy rate. No maternal complications were reported. We found a very different protein profile in implantative cycles, the majority of proteins being down-regulated. This probably reflects a different endometrial functional status, more favourable to implantation. EFA proteomic analysis could be a useful tool in the planning ET strategies. |
| Databáze: | OpenAIRE |
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