Aspects of sterol metabolism in the yeastSaccharomyces cerevisiae and inPhytophthora
| Autor: | J. R. Ramp, F. R. Taylor, R. Gonzales, L. W. Parks, C. K. Bottema, Colleen A. McLean-Bowen, B. W. Jensen |
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| Rok vydání: | 1982 |
| Předmět: |
Phytophthora
Nystatin Membrane permeability Kinetics Mutant Saccharomyces cerevisiae Biology Biochemistry Membrane Potentials chemistry.chemical_compound Lipid bilayer phase behavior Ergosterol Calorimetry Differential Scanning Cell Membrane Organic Chemistry Fungi Cell Biology Yeast Sterol Mitochondria Sterols chemistry Mutation Spectrophotometry Ultraviolet lipids (amino acids peptides and proteins) Fluorescence anisotropy |
| Zdroj: | Lipids. 17:187-196 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/bf02535102 |
| Popis: | Using 5 wild-type strains of yeast, nonequivalence in the isolation of sterol mutants was observed. Experiments are described on the effects of sterol modifications on growth, physical and enzymic properties ofSaccharomyces cerevisiae andPhytophthora cactorum. Although discontinuities in Arrhenius kinetics were observed by fluorescence anisotropy and enzymic measurements of mutants (but not wild-types) of yeast, evidence based on membrane permeability and differential scanning calorimetry failed to support bulk lipid phase transitions as the cause for the discontinuities. |
| Databáze: | OpenAIRE |
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