Multireplicon genome architecture of Lactobacillus salivarius
| Autor: | Julian Parkhill, J. Kevin Collins, Des Higgins, Fergus Shanahan, Gerald F. Fitzgerald, Marcus J. Claesson, Gerald C. O'Sullivan, Ana Cerdeño-Tárraga, Paul W. O'Toole, Jan-Peter van Pijkeren, Carlos Canchaya, Yin Li, Sarah Flynn, Douwe van Sinderen, Sinead C. Leahy |
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| Rok vydání: | 2006 |
| Předmět: |
Pseudogene
Auxotrophy Molecular Sequence Data Locus (genetics) Peptidoglycan Biology Genome Microbiology Pentose Phosphate Pathway Plasmid Amino Acids Gene Genetics Whole genome sequencing Multidisciplinary Lactobacillus salivarius Polysaccharides Bacterial food and beverages Biological Sciences biology.organism_classification Electrophoresis Gel Pulsed-Field Lactobacillus DNA Transposable Elements Carbohydrate Metabolism Replicon Genome Bacterial Pseudogenes Plasmids |
| Zdroj: | Proceedings of the National Academy of Sciences. 103:6718-6723 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.0511060103 |
| Popis: | Lactobacillus salivarius subsp. salivarius strain UCC118 is a bacteriocin-producing strain with probiotic characteristics. The 2.13-Mb genome was shown by sequencing to comprise a 1.83 Mb chromosome, a 242-kb megaplasmid (pMP118), and two smaller plasmids. Megaplasmids previously have not been characterized in lactic acid bacteria or intestinal lactobacilli. Annotation of the genome sequence indicated an intermediate level of auxotrophy compared with other sequenced lactobacilli. No single-copy essential genes were located on the megaplasmid. However, contingency amino acid metabolism genes and carbohydrate utilization genes, including two genes for completion of the pentose phosphate pathway, were megaplasmid encoded. The megaplasmid also harbored genes for the Abp118 bacteriocin, a bile salt hydrolase, a presumptive conjugation locus, and other genes potentially relevant for probiotic properties. Two subspecies of L. salivarius are recognized, salivarius and salicinius , and we detected megaplasmids in both subspecies by pulsed-field gel electrophoresis of sizes ranging from 100 kb to 380 kb. The discovery of megaplasmids of widely varying size in L. salivarius suggests a possible mechanism for genome expansion or contraction to adapt to different environments. |
| Databáze: | OpenAIRE |
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