Measurement of plasma free steroids by direct radioimmunoassay of ultrafiltrate in association with the monitoring of free components with [14C]glucose
| Autor: | Keigo Yasuda, Kiyoshi Miura, Noriyoshi Yamakita, Yoshiyuki Fuwa, Kayoko Adachi, Eiichi Goshima |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Blood Glucose Male 14c glucose Adolescent Hydrocortisone Prednisolone medicine.medical_treatment Clinical Biochemistry Radioimmunoassay Ultrafiltration Endocrine System Diseases Biochemistry Steroid Pregnancy Blood plasma medicine Humans Testosterone Equilibrium dialysis Aged Chromatography Estradiol Chemistry Biochemistry (medical) General Medicine Plasma Middle Aged Ultrafiltration (renal) Female Steroids Dialysis |
| Zdroj: | Clinica Chimica Acta. 200:13-22 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/0009-8981(91)90329-b |
| Popis: | We describe a modified method of centrifugal ultrafiltration using the Grace MPS-3 device for the measurement of plasma free (unbound) steroids (cortisol, testosterone, estradiol, and prednisolone). Plasma was incubated with [14C]glucose to monitor the movement of free components, applied to the MPS-3 and centrifuged. Steroid concentration of ultrafiltrate was directly measured by radioimmunoassay, and multiplied by the ratio of [14C]glucose count (dpm) in plasma to [14C]glucose count (dpm) in ultrafiltrate. The data by this method correlated well with those obtained by equilibrium dialysis. Our results of free steroid in healthy volunteers and patients with various diseases were comparable with the previously reported values. This procedure showed the advantages of small sample volume, rapid separation and the ability to process a large number of samples in a single run. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|