A new model of sperm nuclear architecture following assessment of the organization of centromeres and telomeres in three-dimensions
| Autor: | Nicole M. Millan, Dimitrios Ioannou, Helen G. Tempest, Elizabeth Jordan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Centromere Biology Models Biological Genome Article Nuclear architecture 03 medical and health sciences 0302 clinical medicine Sperm cell medicine Humans In Situ Hybridization Fluorescence Cell Nucleus Genetics 030219 obstetrics & reproductive medicine Multidisciplinary Extramural Middle Aged Telomere Spermatozoa Sperm 030104 developmental biology medicine.anatomical_structure Evolutionary biology Nucleus |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep41585 |
| Popis: | The organization of chromosomes in sperm nuclei has been proposed to possess a unique “hairpin-loop” arrangement, which is hypothesized to aid in the ordered exodus of the paternal genome following fertilization. This study simultaneously assessed the 3D and 2D radial and longitudinal organization of telomeres, centromeres, and investigated whether chromosomes formed the same centromere clusters in sperm cells. Reproducible radial and longitudinal non-random organization was observed for all investigated loci using both 3D and 2D approaches in multiple subjects. We report novel findings, with telomeres and centromeres being localized throughout the nucleus but demonstrating roughly a 1:1 distribution in the nuclear periphery and the intermediate regions with |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|