Differential Spatio-Temporal Regulation of T-Box Gene Expression by microRNAs during Cardiac Development
| Autor: | Diego Franco, Mohamad Alzein, Francisco Hernández-Torres, Amelia Aránega, Carlos García-Padilla, Jorge N. Domínguez, Estefanía Lozano-Velasco |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Untranslated region cardiac development Context (language use) Computational biology Biology Article 03 medical and health sciences 0302 clinical medicine Gene expression microRNA Diseases of the circulatory (Cardiovascular) system Pharmacology (medical) cardiovascular diseases General Pharmacology Toxicology and Pharmaceutics Post-transcriptional regulation Gene T-box genes Gastrulation 030104 developmental biology T-box 030220 oncology & carcinogenesis RC666-701 cardiovascular system post-transcriptional regulation |
| Zdroj: | Journal of Cardiovascular Development and Disease Volume 8 Issue 5 Pages: 56 Journal of Cardiovascular Development and Disease, Vol 8, Iss 56, p 56 (2021) |
| ISSN: | 2308-3425 |
| Popis: | Cardiovascular development is a complex process that starts with the formation of symmetrically located precardiac mesodermal precursors soon after gastrulation and is completed with the formation of a four-chambered heart with distinct inlet and outlet connections. Multiple transcriptional inputs are required to provide adequate regional identity to the forming atrial and ventricular chambers as well as their flanking regions; i.e., inflow tract, atrioventricular canal, and outflow tract. In this context, regional chamber identity is widely governed by regional activation of distinct T-box family members. Over the last decade, novel layers of gene regulatory mechanisms have been discovered with the identification of non-coding RNAs. microRNAs represent the most well-studied subcategory among short non-coding RNAs. In this study, we sought to investigate the functional role of distinct microRNAs that are predicted to target T-box family members. Our data demonstrated a highly dynamic expression of distinct microRNAs and T-box family members during cardiogenesis, revealing a relatively large subset of complementary and similar microRNA–mRNA expression profiles. Over-expression analyses demonstrated that a given microRNA can distinctly regulate the same T-box family member in distinct cardiac regions and within distinct temporal frameworks, supporting the notion of indirect regulatory mechanisms, and dual luciferase assays on Tbx2, Tbx3 and Tbx5 3′ UTR further supported this notion. Overall, our data demonstrated a highly dynamic microRNA and T-box family members expression during cardiogenesis and supported the notion that such microRNAs indirectly regulate the T-box family members in a tissue- and time-dependent manner. |
| Databáze: | OpenAIRE |
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