Restriction enzymes and their use in molecular biology: An overview
| Autor: | Giorgio Camilloni, Francesca Di Felice, Gioacchino Micheli |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
MOLECULAR BIOLOGY METHODS Biology History 21st Century 01 natural sciences General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Dna genetics Transcription Activator-Like Effector Nucleases Humans Cloning Molecular Molecular Biology Gene Nucleotide Mapping Chromosome Mapping DNA DNA Restriction Enzymes General Medicine DNA Methylation History 20th Century Molecular biology Chromatin humanities Nobel Prize DNA metabolism Restriction enzyme chemistry Cloning DNA manipulation history restriction enzymes CRISPR-Cas Systems General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Journal of Biosciences. 44 |
| ISSN: | 0973-7138 0250-5991 |
| Popis: | Restriction enzymes have been identified in the early 1950s of the past century and have quickly become key players in the molecular biology of DNA. Forty years ago, the scientists whose pioneering work had explored the activity and sequence specificity of these enzymes, contributing to the definition of their enormous potential as tools for DNA characterization, mapping and manipulation, were awarded the Nobel Prize. In this short review, we celebrate the history of these enzymes in the light of their many different uses, as these proteins have accompanied the history of DNA for over 50 years representing active witnesses of major steps in the field. |
| Databáze: | OpenAIRE |
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