Characterization of a polysaccharide from Eupolyphaga sinensis walker and its effective antitumor activity via lymphocyte activation

Autor: Changlin Zhou, Jialu Ding, Baiyong Shen, Lingman Ma, Wen Shen, Duiyue Xu, Liangyuan He, Y.H. Zhou, Xin Xie
Rok vydání: 2020
Předmět:
Arabinose
Magnetic Resonance Spectroscopy
Cockroaches
02 engineering and technology
Lymphocyte proliferation
Lymphocyte Activation
Rhamnose
Biochemistry
Fucose
Eupolyphaga sinensis
Mice
chemistry.chemical_compound
fluids and secretions
Structural Biology
chemistry.chemical_classification
Mice
Inbred BALB C
0303 health sciences
Xylose
biology
GalP
Liver Neoplasms
General Medicine
Chromatography
Ion Exchange
021001 nanoscience & nanotechnology
Killer Cells
Natural
Chromatography
Gel
Female
0210 nano-technology
Carcinoma
Hepatocellular
Cell Survival
Antineoplastic Agents
Polysaccharide
03 medical and health sciences
Polysaccharides
Cell Line
Tumor
Animals
Molecular Biology
Cell Proliferation
030304 developmental biology
Galactose
biochemical phenomena
metabolism
and nutrition
biology.organism_classification
Glucose
chemistry
biology.protein
Zdroj: International Journal of Biological Macromolecules. 162:31-42
ISSN: 0141-8130
DOI: 10.1016/j.ijbiomac.2020.06.120
Popis: A polysaccharide (ESPS) purified from Eupolyphaga sinensis Walker by ion exchange chromatography and gel chromatography was investigated, including its structure characterization and antitumor activity. The results showed that ESPS was composed of rhamnose, fucose, arabinose, xylose, glucose, and galactose in a molar ratio of 7.4: 3.1: 13.9: 9.3: 39.7: 26.5, with the mean weight (Mw) of 2.14 × 104 Da; the main chain of ESPS was mainly composed of → 4) - α - D - Glcp - (1 → and → 3) - β - D - Galp - (1 →, and the side chains were connected to the main chain through the O-6 atom of glucose and O-4 and O-6 atom of galactose. In addition, ESPS promoted the lymphocyte proliferation and inhibited liver cancer cells growth through enhancing lymphocyte activity in vitro, mainly NK cells. Moreover, ESPS markedly stimulated immunity in H22-bearing mice by increasing the spleen and thymus indices and effectively inhibited H22 cell growth in vivo. These data indicated that ESPS was a polysaccharide component possessing high anti-hepatocellular carcinoma activity, representing a potential immunotherapy candidate for the treatment of liver cancer.
Databáze: OpenAIRE
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