MicroRNA-140-5p aggravates hypertension and oxidative stress of atherosclerosis via targeting Nrf2 and Sirt2
| Autor: | Ke Ren, Qing‑Quan Liu, Chang‑Jun Huang, Su‑Hong Liu, Wei‑Min Li, Xiu‑Hui Yang |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male NF-E2-Related Factor 2 microRNA-140-5p 030204 cardiovascular system & hematology SIRT2 medicine.disease_cause sirtuin 2 Cell Line 03 medical and health sciences Mice 0302 clinical medicine Downregulation and upregulation Western blot Genetics medicine Human Umbilical Vein Endothelial Cells Animals Humans Cells Cultured chemistry.chemical_classification reactive oxygen species Reactive oxygen species biology medicine.diagnostic_test Chemistry General Medicine Articles Atherosclerosis KEAP1 Molecular biology Heme oxygenase MicroRNAs Oxidative Stress 030104 developmental biology nuclear factor erythroid 2-related factor 2 Sirtuin Hypertension biology.protein Oxidative stress Heme Oxygenase-1 |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X |
| Popis: | In the present study, the function of microRNA (miR)‑140‑5p on oxidative stress in mice with atherosclerosis was investigated. A reverse transcription‑quantitative polymerase chain reaction assay was used to determine the expression of miR‑140‑5p. Oxidative stress kits and reactive oxygen species (ROS) kits were used to analyze alterations in oxidative stress and ROS levels. The alterations in protein expression were determined using western blot analysis and an immunofluorescence assay. miR‑140‑5p expression was increased in mice with atherosclerosis with hypertension. Consistently, miR‑140‑5p expression was also increased in mice with atherosclerosis. Upregulation of miR‑140‑5p increased oxidative stress and ROS levels by suppressing the protein expression of nuclear factor erythroid 2‑related factor 2 (Nrf2), sirtuin 2 (Sirt2), Kelch‑like enoyl‑CoA hydratase‑associated protein 1 (Keap1) and heme oxygenase 1 (HO‑1) in vitro. By contrast, downregulation of miR‑140‑5p decreased oxidative stress and ROS levels by activating the protein expression of Nrf2, Sirt2, Keap1 and HO‑1 in vitro. Sirt2 agonist or Nrf2 agonist inhibited the effects of miR‑140‑5p on oxidative stress in vitro. Collectively, these results suggested that miR‑140‑5p aggravated hypertension and oxidative stress of mice with atherosclerosis by targeting Nrf2 and Sirt2. |
| Databáze: | OpenAIRE |
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