Modulation of immunological responses by aqueous extract of Datura stramonium L. seeds on cyclophosphamide-induced immunosuppression in Wistar rats
| Autor: | Arome Solomon Odiba, Joyce Oloaigbe Ogidigo, Parker Elijah Joshua, Rita Onyekachukwu Asomadu, Daniel Emmanuel Ekpo, Yahaya Junaidu, Samson Ayodeji Oka, Olasupo Stephen Adeniyi |
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| Rok vydání: | 2022 |
| Předmět: |
Datura stramonium
Cyclophosphamide medicine.medical_treatment Immunology Pharmacology Antioxidants Catechin Gallic Acid medicine Animals Rats Wistar Luteolin Immunosuppression Therapy Vanillic Acid Aqueous extract Glutathione Peroxidase Quinine biology Plant Extracts Superoxide Dismutase Chemistry Water Immunosuppression Vitamins Saponins Catalase biology.organism_classification Rats Immunoglobulin A Levamisole Immunoglobulin G Seeds Quercetin Chlorogenic Acid Lipoproteins HDL Tannins Limonene medicine.drug |
| Zdroj: | BMC Immunology. 23 |
| ISSN: | 1471-2172 |
| Popis: | Background Datura stramonium L. (Solanaceae) is used traditionally in west Africa to treat asthma, epilepsy, rheumatoid arthritis, filariasis microbial infections and conjunctivitis. This study investigated the immunomodulatory effects of aqueous seed extract of D. stramonium L. (ASEDS) on Wistar rats. Methods Thirty Wistar albino rats (180–200 g) were randomized into 6 groups (n = 5). Group 1 received distilled water only. Rats in groups 2–6 were pretreated with 10 mg/kg body weight (b.w.) Cyclophosphamide orally for 27-days to induce immunosuppression. Thereafter, they received treatment orally for 28 days as follows: Group 2 (distilled water), group 3 (5 mg/kg b.w. Levamisole), groups 4–6 (60, 90 and 120 mg/kg b.w. ASEDS, respectively). HPLC was used to determine major compounds in ASEDS. The effects of ASEDS on immune cells, immunoglobulins A, G and M levels, lipoproteins, and antioxidant status of rats were evaluated. Results ASEDS indicated high content of Acutumine, Quinine, Catechin, Chlorogenic acid, Gallic acid, Quercetin, Vanillic acid, Luteolin, Formosanin C, Saponin, Cyanidin, Tannic acid, 3-Carene, Limonene and α-terpineol. Cyclophosphamide triggered significant (p p Conclusions The results obtained from the study showed the immunomodulatory activity of ASEDS, thereby indicating its potential in immunostimulatory drug discovery. |
| Databáze: | OpenAIRE |
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