Variations in bacterial community structure and antimicrobial resistance gene abundance in cattle manure and poultry litter
| Autor: | Yichao Yang, Stefania Cocco, Mary C. Savin, Biyensa Gurmessa, Steven C. Ricke, Ester Foppa Pedretti, Philip A. Moore, Giuseppe Corti, Amanda J. Ashworth |
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| Rok vydání: | 2020 |
| Předmět: |
Veterinary medicine
010501 environmental sciences Biology 01 natural sciences Biochemistry Poultry 03 medical and health sciences Soil 0302 clinical medicine Nutrient Antibiotic resistance Drug Resistance Bacterial Animals 030212 general & internal medicine Poultry litter Illumina dye sequencing 0105 earth and related environmental sciences General Environmental Science Community structure Manure Anti-Bacterial Agents Genes Bacterial Composition (visual arts) Alpha diversity Cattle |
| Zdroj: | Environmental research. 197 |
| ISSN: | 1096-0953 |
| Popis: | Cattle manure and poultry litter are widely used as fertilizers as they are excellent sources of nutrients; however, potential adverse environmental effects exist during land applications, due to the release of zoonotic bacteria and antimicrobial resistance (AMR) genes. This study was conducted to understand linkages between physiochemical composition, bacterial diversity, and AMR gene presence of cattle manure and poultry litter using quantitative polymerase chain reaction to enumerate four AMR genes (ermB, sulI, intlI, and blactx-m-32), Illumina sequencing of the 16 S region, and analysis of physical and chemical properties. Principal coordinate analysis of Bray–Curtis distance revealed distinct bacterial community structures between the two manure sources. Greater alpha diversity occurred in cattle manure compared to poultry litter (P |
| Databáze: | OpenAIRE |
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