Effect of tumor necrosis factor-α and interleukin-1α on heme oxygenase-1 expression in human endothelial cells
| Autor: | Jennifer A. Clikeman, John R. Hoidal, Karleen S. Callahan, Christi M. Terry |
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| Rok vydání: | 1998 |
| Předmět: |
Transcription
Genetic Endothelium Physiology medicine.medical_treatment Blotting Western Inflammation Biology medicine.disease_cause Gene Expression Regulation Enzymologic chemistry.chemical_compound Physiology (medical) medicine Humans RNA Messenger Heme Cells Cultured Protein Synthesis Inhibitors Interleukin-6 Tumor Necrosis Factor-alpha Molecular biology Endothelial stem cell Heme oxygenase medicine.anatomical_structure Cytokine chemistry Biochemistry Cell culture Heme Oxygenase (Decyclizing) Dactinomycin Endothelium Vascular medicine.symptom Cardiology and Cardiovascular Medicine Oxidative stress Interleukin-1 |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 274:H883-H891 |
| ISSN: | 1522-1539 0363-6135 |
| DOI: | 10.1152/ajpheart.1998.274.3.h883 |
| Popis: | Heme iron exacerbates oxidant damage by catalyzing the production of free radicals. Heme oxygenase is the rate-limiting enzyme involved in heme catabolism. An inducible form of heme oxygenase, heme oxygenase-1 (HO-1), is upregulated in oxidant and inflammatory settings, and recent work suggests that HO-1 induction may serve a protective function against oxidant injury. The ability of the endogenous inflammatory mediators, interleukin (IL)-1 alpha, tumor necrosis factor-alpha (TNF-alpha), and IL-6, to enhance HO-1 expression in cultured human endothelial cells was examined in this study. HO-1 mRNA and protein expression were upregulated by IL-1 alpha and TNF-alpha exposure but not by IL-6. Induction of HO-1 mRNA by IL-1 alpha and TNF-alpha occurred in a concentration- and time-dependent fashion, with maximal expression occurring by 4 h for both cytokines. Induction depended on protein synthesis and occurred at the transcriptional level. Inhibition of the AP-1 transcription factor with curcumin decreased the cytokine induction of HO-1 mRNA, suggesting the involvement of this transcription factor in cytokine signaling of HO-1. The results of this study indicate that the endogenous inflammatory cytokines IL-1 alpha and TNF-alpha induce HO-1 in endothelial cells, providing further evidence that HO-1 may be an important cellular response to inflammatory stress. |
| Databáze: | OpenAIRE |
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