Production and Characterization of a Novel Monoclonal Antibody Inhibitory for Murine Natural Killer Cell Activity
| Autor: | David W. Hoskin, J. C. Roder |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Cytotoxicity
Immunologic Male T-Lymphocytes Immunoblotting Immunology Enzyme-Linked Immunosorbent Assay Biology CD49b Natural killer cell Mice Interleukin 21 NK-92 Antibody Specificity Tumor Cells Cultured medicine Animals Cytotoxic T cell Mice Inbred C3H Hybridomas Lymphokine-activated killer cell Cell Membrane Antibodies Monoclonal Rats Inbred Strains General Medicine Natural killer T cell Molecular biology Rats Killer Cells Natural medicine.anatomical_structure Mice Inbred DBA Antigens Surface Mice Inbred CBA Interleukin 12 T-Lymphocytes Cytotoxic |
| Zdroj: | Immunological Investigations. 21:11-23 |
| ISSN: | 1532-4311 0882-0139 |
| DOI: | 10.3109/08820139209069359 |
| Popis: | Natural killer (NK) cells are considered to play an important role in tumor surveillance. The killing of tumor target cells by NK cells is the result of a complex series of sequential binding, signal processing and lytic events. However, the mechanism which NK cells use to recognize tumor targets is poorly understood. To further study the cell-surface molecules involved in tumor recognition, we immunized rats against cloned murine T cells with NK activity (DBA/2.1) and generated rat-mouse hybridomas which were screened for the ability to block lytic activity of DBA/2.1 effector cells. Culture supernatants from one IgM-producing hybridoma, designated S1C4, were found to consistently inhibit DBA/2.1-mediated lysis of YAC-1 target cells. Endogenous splenic NK activity was also diminished in the presence of S1C4 monoclonal antibody (mAb) while alloantigen-specific cytotoxic T lymphocyte (CTL) activity was not affected. S1C4 mAb appears to react with effector cell-surface structures involved in the recognition/adhesion phase of NK activity since pretreatment of effector cells with mAb S1C4 inhibits their ability to bind to YAC-1 target cells. ELISA studies revealed that the S1C4 antigen is expressed by a range of lymphoid cell lines, as well as by DBA/2.1 cells and fresh splenic NK cells. S1C4 mAb were shown to react with 22, 24, 30, and 46 kiloDalton (kDa) DBA/2.1 cell membrane components on immunoblots performed under reducing conditions. These structures do not correspond to any known recognition/adhesion molecules, suggesting that mAb S1C4 defines novel cell membrane components involved in NK cell function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|