A quantitative UHPLC-MS/MS method for the growth hormone-releasing peptide-6 determination in complex biological matrices and transdermal formulations
Autor: | Sylvie Marleau, Catherine Michaud, Davide Brambilla, Huy Ong, Sébastien Sauvé, Xavier Banquy, Cloé L. Esposito, Elise Laszlo, Sung Vo Duy, Araceli Garcia Ac |
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Přispěvatelé: | Université de Montréal. Faculté de pharmacie |
Rok vydání: | 2021 |
Předmět: |
Calibration curve
Swine Extraction Peptide 02 engineering and technology 01 natural sciences Uhplc ms ms Analytical Chemistry Plasma Liquid chromatography–mass spectrometry Limit of Detection Tandem Mass Spectrometry Transdermal drug delivery Animals Chromatography High Pressure Liquid Skin Transdermal Detection limit chemistry.chemical_classification Chromatography Chemistry 010401 analytical chemistry Extraction (chemistry) Reproducibility of Results Permeation 021001 nanoscience & nanotechnology 0104 chemical sciences UHPLC-MS/MS 0210 nano-technology Oligopeptides Chromatography Liquid |
Zdroj: | Talanta. 233 |
ISSN: | 1873-3573 |
Popis: | Growth hormone-releasing peptide-6 (GHRP-6) is part of a group of small synthetic peptides with potent GH-releasing activity that have gained attention in the last two decades by virtue of their cyto- and cardioprotective effects. Despite numerous preclinical studies highlighting the potential cardiovascular benefits of GHRP-6, confirmation of clinical efficacy is still awaited. Recent advances in transdermal drug delivery systems have been made to address challenges related to the poor skin permeation rate of peptides by using pain-free microneedle (MN) devices. Accordingly, highly sensitive and validated analytical methods are required for the potential clinical translation of MN-based peptides. The ultra-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) methods developed in this study aimed to quantify GHRP-6 in biological matrices (plasma, skin) and dissolving polymeric MNs. UHPLC/MS-MS method detection limits of 0.1, 1.1, 0.9 and 1.5 ng/mL were achieved in neat solution, plasma, MN polymer solution, and skin matrices, respectively. Method validation also involved assessment of precision, accuracy, limits of quantification, linearity of matched calibration curves (R2 > 0.990), extraction recovery, matrix effect, stability studies, selectivity, and carry-over effect. Additionally, quality control samples were analyzed at three concentration levels to determine recovery (85–109%) and accuracy/bias (3.2–14.7%). Intra- and inter-day precision were within the range of acceptance (RSDs of 3.0–13.9% and 0.4–14.5%, respectively). The validity and applicability of such methods were successfully demonstrated for transdermal GHRP-6 delivery using GHRP-6-loaded MN patches applied to pig skin. |
Databáze: | OpenAIRE |
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