Inhibitory effects of Leuconostoc mesenteroides 1RM3 isolated from narezushi on lipopolysaccharide-induced inflammation in RAW264.7 mouse macrophage cells and dextran sodium sulphate-induced inflammatory bowel disease in mice
| Autor: | Bon Kimura, Takashi Kuda, Hajime Takahashi, Kanno Tomomi, Miho Kawahara |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharide
IBD Medicine (miscellaneous) Inflammation Leuconostoc mesenteroides Nitric oxide Microbiology Mice chemistry.chemical_compound Immune system Medicine Macrophage TX341-641 Cytotoxicity Nutrition and Dietetics biology Nutrition. Foods and food supply business.industry Fermented fish RAW264.7 biology.organism_classification digestive system diseases Dextran chemistry Antioxidant medicine.symptom business Food Science |
| Zdroj: | Journal of Functional Foods, Vol 6, Iss, Pp 631-636 (2014) |
| ISSN: | 1756-4646 |
| DOI: | 10.1016/j.jff.2013.11.017 |
| Popis: | The anti-inflammatory bowel disease (anti-IBD) activity of Leuconostoc mesenteroides 1RM3 isolated from saba-narezushi , which has known antioxidant properties, was examined by monitoring its immune and anti-inflammatory activities on murine macrophage RAW264.7 and dextran sodium sulphate (DSS)-induced IBD model mice. Heat-killed Ln. mesenteroides 1RM3 cells promoted nitric oxide (NO) generation in intact RAW264.7 cells. Ln. mesenteroides 1RM3 cells suppressed Escherichia coli O111 lipopolysaccharide (LPS)-induced NO generation. This bacterium also significantly protected RAW264.7 cells from cytotoxicity due to exposure to H 2 O 2 . In the case of mice administered with drinking water containing 5% (w/v) DSS, Ln. mesenteroides 1RM3 (about 9.7 log CFU/ml) suppressed colon inflammation, atrophy, diarrheal and bloody bowl discharge. These results suggest that Ln. mesenteroides 1RM3 could be used as probiotics to improve IBD. |
| Databáze: | OpenAIRE |
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