Accessible and Validated Processing of SARS-CoV-2 from Wastewater
| Autor: | Nicole C. Rondeau, JJ L. Miranda, Lina A. Ariyan, Oliver J. Rose, Brian J. Mailloux |
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| Rok vydání: | 2021 |
| Předmět: |
2019-20 coronavirus outbreak
Coronavirus disease 2019 (COVID-19) Computer science Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses virus diseases Computational biology Protocols and Workflows Immunology and Microbiology (miscellaneous) Wastewater Genetics Viral rna skin and connective tissue diseases Molecular Biology |
| Zdroj: | Microbiology Resource Announcements |
| ISSN: | 2576-098X |
| Popis: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of coronavirus disease 2019 (COVID-19), is shed into wastewater. Accessible methods are necessary for processing samples in a myriad of contexts. We optimized a protocol for extracting viral RNA for downstream experiments. Our pipeline was validated with SARS-CoV-2 itself as a matrix recovery and quantitative measurement control. |
| Databáze: | OpenAIRE |
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