High-throughput Evaluation of Protein Migration and Localization after Laser Micro-Irradiation
| Autor: | Adam Krysztofiak, Audrey Turchick, Sebastian Oeck, Nathalie M. Malewicz, Verena Jendrossek, Peter M. Glazer |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
DNA Repair DNA damage DNA repair Cell Medizin lcsh:Medicine Article Histones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Humans DNA Breaks Double-Stranded Phosphorylation lcsh:Science Multidisciplinary biology Chemistry Lasers lcsh:R Proteins Fluorescence Histone Code 030104 developmental biology Histone medicine.anatomical_structure Histone phosphorylation Microscopy Fluorescence biology.protein Biophysics lcsh:Q Single-Cell Analysis Protein Processing Post-Translational Quantitative analysis (chemistry) 030217 neurology & neurosurgery DNA DNA Damage |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-39760-8 |
| Popis: | DNA- and histone-related research frequently comprises the quantitative analysis of protein modifications, such as histone phosphorylation. Analysis of accumulation and disappearance of protein foci are used to monitor DNA damage and repair kinetics. If the protein of interest doesn’t accumulate in foci, laser micro-irradiation of single nuclei provides an alternative method to monitor DNA repair proteins and histone dynamics at the DNA damage site. We have developed an automated evaluation tool for standardized, high-throughput analysis of micro-irradiated cells featuring single cell background subtraction and detection across multiple fluorescence channels, allowing for robust statistics. |
| Databáze: | OpenAIRE |
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