Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro
| Autor: | Kenneth J. Spolnik, Beatriz Helena Dias Panariello, Justin K Kindler, George J. Eckert, Simone Duarte, Ygal Ehrlich |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.medical_specialty
Sodium Hypochlorite Root canal medicine.medical_treatment Science Dentistry Dental diseases In Vitro Techniques Enterococcus faecalis Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Humans Saline Colony-forming unit Multidisciplinary biology business.industry Electromagnetic Radiation Biofilm 030206 dentistry biology.organism_classification Endodontics In vitro Root Canal Therapy Disinfection medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Sodium hypochlorite Biofilms Medicine Saline Solution Dental Pulp Cavity business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2045-2322 |
| Popis: | Root canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for the disinfection of root canal in vitro, single rooted teeth were instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with Enterococcus faecalis ATCC 29,212, which grew for 15 days to form an established biofilm. Samples were treated with 6% sodium hypochlorite (NaOCl), 1.5% NaOCl 1.5% NaOCl with EMS, 0.9% saline with EMS or 0.9% saline. After treatments, the colony forming units (CFU) was determined. Data was analyzed by Wilcoxon Rank Sums Test (α = 0.05). One sample per group was scored and split for confocal laser scanning microscopy imaging. There was a significant effect with the use of NaOCl with or without EMS versus 0.9% saline with or without EMS (p = 0.012 and 0.003, respectively). CFUs were lower when using 0.9% saline with EMS versus 0.9% saline alone (p = 0.002). Confocal imaging confirmed CFU findings. EMS with saline has an antibiofilm effect against E. faecalis and can potentially be applied for endodontic disinfection. |
| Databáze: | OpenAIRE |
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