The effect of Jiedu Huoxue decoction on rat model of experimental nonbacterial prostatitis via regulation of miRNAs

Autor: Wang Wanchun, Wang Jiangang, Wenli Mao, Yunbo Wu, Yan Zhangren, Gang Huang, Chunhua Huang, Yi Jun
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
Rat model
Pharmaceutical Science
Prostatitis
Differentially expressed mirnas
Decoction
target gene prediction
RM1-950
Pharmacology
second-generation sequencing
030226 pharmacology & pharmacy
01 natural sciences
Lethal Dose 50
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Drug Discovery
microRNA
medicine
Animals
Dose-Response Relationship
Drug
business.industry
Polydatin
differentially expressed miRNAs
High-Throughput Nucleotide Sequencing
General Medicine
functional enrichment analysis
medicine.disease
0104 chemical sciences
Rats
010404 medicinal & biomolecular chemistry
Disease Models
Animal
MicroRNAs
Complementary and alternative medicine
Potential biomarkers
Chronic Disease
Molecular Medicine
Therapeutics. Pharmacology
business
Research Article
Drugs
Chinese Herbal
Zdroj: Pharmaceutical Biology
article-version (VoR) Version of Record
Pharmaceutical Biology, Vol 58, Iss 1, Pp 745-759 (2020)
ISSN: 1744-5116
1388-0209
Popis: Context The underlying mechanisms of Jiedu Huoxue decoction (JDHXD) in treating chronic prostatitis have not been fully explored. Objective This study investigates the miRNAs as potential biomarkers and the effect of JDHXD on the rat model of experimental nonbacterial prostatitis. Materials and methods Fifty-four Sprague-Dawley male rats were randomly divided into normal control, model, JDHXD low dose (0.5 g/kg/day), medium dose (1 g/kg/day), high dose (2 g/kg/day) and western medicine (cernilton 0.094 g/kg/day) groups, and intragastrically administered once daily for 30 days. The control and model (upon successful establishment) groups received distilled water. Differential expression of miRNAs was analysed with high-throughput miRNA sequencing and validated with qRT-PCR and Northern blot. Prediction of specific target genes and functional enrichment analysis were performed with bioinformatics. Results LD50 test showed no sign of toxicity with maximum feasible dose 4 g/kg JDHXD. Compared with control, 495 miRNAs showed expression changes in CAP/CPPS rats, of which 211 were significantly different and 37 were prostatic-related. There were 181 differentially expressed miRNAs between the model and high dose JDHXD groups, of which 23 were identical with the control and model groups. Compared with control, miR-146a, miR-423 and miR-205 expression increased significantly in the model group, decreased dose-dependently in the JDHXD groups (p 0.05). Discussion and conclusions Future studies may explore the contributions of the active components in JDHXD. The study design is generalisable. The effect can be repeatedly verified in clinical trials.
Databáze: OpenAIRE
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