Molecular markers for discrimination of benign and malignant follicular thyroid tumors
| Autor: | Theodoros Foukakis, Anders Höög, Jia-Jing Lee, Hanna Göransson, Göran Wallin, Anders Isaksson, Ulf Landegren, Catharina Larsson, Ulf Pettersson, Mats G. Gustafsson, Mårten Fryknäs, Ulrika Wickenberg-Bolin, Lars Grimelius |
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| Rok vydání: | 2005 |
| Předmět: |
Adenoma
Adult Genetic Markers Male endocrine system Pathology medicine.medical_specialty endocrine system diseases CDNA Arrays Biology Polymerase Chain Reaction Diagnosis Differential Follicular phase Adenocarcinoma Follicular medicine Humans Neoplasm Invasiveness Thyroid Neoplasms Thyroid tumors Aged DNA Primers Oligonucleotide Array Sequence Analysis Thyroid General Medicine Follicular Adenomas Middle Aged medicine.disease Thyroid Diseases medicine.anatomical_structure Adenocarcinoma Female |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 27(4) |
| ISSN: | 1010-4283 |
| Popis: | To identify molecular markers useful for the diagnostic discrimination of benign and malignant follicular thyroid tumors.A panel of thyroid tumors was characterized with expression profiling using cDNA microarrays. A robust algorithm for gene selection was developed to identify molecular markers useful for the classification of heterogeneous tumor classes. The study included tumor tissue specimens from 10 patients with benign follicular adenomas and from 10 with malignant tumors. The malignant tumors mainly consisted of clinically relevant minimally invasive follicular carcinomas. The mRNA expression level of a candidate gene, FHL1, was evaluated in an independent series of 61 tumors.22 gene expression markers were identified as differentially expressed. Several of the identified genes, for example DIO1, CITED1, CA12 and FN1, have previously been observed as differentially expressed in various thyroid tumors. FHL1 was significantly underexpressed in carcinomas compared to adenomas in the independent panel of tumors. The results indicate that a small number of genes can be useful to distinguish follicular adenomas from follicular carcinomas.Our findings clearly corroborate previous studies and identify novel candidate molecular markers. These genes have the potential for molecular classification of follicular thyroid tumors and for providing improved understanding of the molecular mechanisms involved in thyroid malignancies. |
| Databáze: | OpenAIRE |
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