MiR-18a-downregulated RORA inhibits the proliferation and tumorigenesis of glioma using the TNF-α-mediated NF-κB signaling pathway
| Autor: | Zhitao Jing, Haiying Zhang, Dan Zou, Ye Zhang, Jiangfeng Hu, Dianqi Hou, Jinpeng Zhou, Junshuang Zhao, Long Li, Yang Jiang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Research paper Retinoic acid lcsh:Medicine medicine.disease_cause RORA chemistry.chemical_compound Mice 0302 clinical medicine 3' Untranslated Regions microRNA-18a Orphan receptor lcsh:R5-920 Cell Cycle NF-kappa B Nuclear Receptor Subfamily 1 Group F Member 1 General Medicine Glioma Middle Aged Prognosis Immunohistochemistry Cell Transformation Neoplastic 030220 oncology & carcinogenesis Female RNA Interference Signal transduction lcsh:Medicine (General) Signal Transduction Adult Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Neurosphere Cell Line Tumor microRNA medicine Biomarkers Tumor Glioma stem cells Animals Humans Viability assay neoplasms Aged Tumor Necrosis Factor-alpha Gene Expression Profiling lcsh:R Computational Biology medicine.disease nervous system diseases Disease Models Animal MicroRNAs 030104 developmental biology chemistry Tumorigenesis Cancer research Carcinogenesis Biomarkers |
| Zdroj: | EBioMedicine EBioMedicine, Vol 52, Iss, Pp-(2020) |
| ISSN: | 2352-3964 |
| Popis: | Background: Glioma has a poor prognosis, and is the most common primary and lethal primary malignant tumor in the central nervous system. Retinoic acid receptor-related orphan receptor A (RORA) is a member of the ROR subfamily of orphan receptors and plays an anti-tumor role in several cancers. Methods: A cell viability assay, the Edu assay, neurosphere formation assay, and xenograft experiments were used to detect the proliferative abilities of glioma cell line, glioma stem cells (GSCs). Western blotting, ELISAs, and luciferase reporter assays were used to detect the presence of possible microRNAs. Findings: Our study found for the first time that RORA was expressed at low levels in gliomas, and was associated with a good prognosis. RORA overexpression inhibited the proliferation and tumorigenesis of glioma cell lines and GSCs via inhibiting the TNF-α mediated NF-κB signaling pathway. In addition, microRNA-18a had a promoting effect on gliomas, and was the possible reason for low RORA expression in gliomas. Interpretation: RORA may be a promising therapeutic target in the treatment of gliomas. Keywords: Glioma, Glioma stem cells, RORA, Tumorigenesis, microRNA-18a |
| Databáze: | OpenAIRE |
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