Loss of GET pathway orthologs in Arabidopsis thaliana causes root hair growth defects and affects SNARE abundance
Autor: | Lisa Yasmin Asseck, Shuping Xing, Alessa Voss, Dietmar G. Mehlhorn, Philipp Denninger, Guido Grossmann, Christopher Grefen, Markus Schwarzländer, Niklas Wallmeroth, Ritwika Kar, Vanessa A. F. Schmidt, York-Dieter Stierhof |
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Rok vydání: | 2017 |
Předmět: |
Organelles
0301 basic medicine Saccharomyces cerevisiae Proteins Multidisciplinary Endoplasmic reticulum Cellular homeostasis Root hair Root hair elongation Biology biology.organism_classification Ribosome Cell biology Protein Transport 03 medical and health sciences 030104 developmental biology PNAS Plus Arabidopsis thaliana Biogenesis Cytokinesis Plant Proteins |
Zdroj: | Proceedings of the National Academy of Sciences. 114 |
ISSN: | 1091-6490 0027-8424 |
Popis: | Next Section Abstract Soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) proteins are key players in cellular trafficking and coordinate vital cellular processes, such as cytokinesis, pathogen defense, and ion transport regulation. With few exceptions, SNAREs are tail-anchored (TA) proteins, bearing a C-terminal hydrophobic domain that is essential for their membrane integration. Recently, the Guided Entry of Tail-anchored proteins (GET) pathway was described in mammalian and yeast cells that serve as a blueprint of TA protein insertion [Schuldiner M, et al. (2008) Cell 134(4):634–645; Stefanovic S, Hegde RS (2007) Cell 128(6):1147–1159]. This pathway consists of six proteins, with the cytosolic ATPase GET3 chaperoning the newly synthesized TA protein posttranslationally from the ribosome to the endoplasmic reticulum (ER) membrane. Structural and biochemical insights confirmed the potential of pathway components to facilitate membrane insertion, but the physiological significance in multicellular organisms remains to be resolved. Our phylogenetic analysis of 37 GET3 orthologs from 18 different species revealed the presence of two different GET3 clades. We identified and analyzed GET pathway components in Arabidopsis thaliana and found reduced root hair elongation in Atget lines, possibly as a result of reduced SNARE biogenesis. Overexpression of AtGET3a in a receptor knockout (KO) results in severe growth defects, suggesting presence of alternative insertion pathways while highlighting an intricate involvement for the GET pathway in cellular homeostasis of plants. |
Databáze: | OpenAIRE |
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