Angiogenin Attenuates Scar Formation in Burn Patients by Reducing Fibroblast Proliferation and Transforming Growth Factor β1 Secretion
| Autor: | Shin Chen Pan, Wei Yu Fang, Chou Hwei Lee, Chung-Lin Chen, Li Wha Wu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Cicatrix Hypertrophic Angiogenin Enzyme-Linked Immunosorbent Assay Smad2 Protein In Vitro Techniques Sensitivity and Specificity Recombinant Angiogenin Cohort Studies Transforming Growth Factor beta1 Neovascularization 030207 dermatology & venereal diseases 03 medical and health sciences Injury Severity Score 0302 clinical medicine medicine Humans Secretion Fibroblast Cells Cultured Cell Proliferation Wound Healing Cell growth business.industry Ribonuclease Pancreatic Fibroblasts 030104 developmental biology medicine.anatomical_structure Cancer research Female Surgery Signal transduction medicine.symptom Burns business Biomarkers Transforming growth factor |
| Zdroj: | Annals of Plastic Surgery. 80:S79-S83 |
| ISSN: | 0148-7043 |
| DOI: | 10.1097/sap.0000000000001306 |
| Popis: | Background Deep burn wounds have a high tendency to form hypertrophic scars. Previously, we found that angiogenin promoted neovascularization during deep burn wound healing. However, the association between angiogenin and scar formation is unclear. Methods We obtained human burn scar tissues from patients who underwent scar surgery and examined the role of angiogenin in scar tissues and determined its effects in scar fibroblasts and on transforming growth factor β1 (TGF-β1) secretion. Results Our results showed an inverse correlation between angiogenin expression and scar severity. Next, we examined the effects of angiogenin in scar fibroblasts. We found that angiogenin was persistently expressed in human scar fibroblasts and that angiogenin expression significantly increased with time in the culture medium of scar fibroblasts. Treatment of scar fibroblasts with recombinant angiogenin significantly decreased their proliferation and TGF-β1 secretion. Moreover, angiogenin inhibited TGF-β1-mediated Smad2 signaling pathway. Conclusion Our data suggest a negative role of angiogenin in fibroblast proliferation via TGF-β1-mediated Smad2 signaling pathway. |
| Databáze: | OpenAIRE |
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