Determination of biogenic amines in fish tissues by ion-exchange chromatography with conductivity detection
| Autor: | F Abballe, F. Longo, A. L. Cinquina, L. de Santis, A. Calı̀, A Severoni |
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| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Cadaverine Chromatography Conductometry Thermal conductivity detector Biogenic Polyamines Organic Chemistry Ion chromatography Reproducibility of Results General Medicine Chromatography Ion Exchange Sensitivity and Specificity Biochemistry Amperometry Analytical Chemistry chemistry.chemical_compound chemistry Biogenic amine Electrochemistry Animals Nitrite Chromatography High Pressure Liquid Histamine |
| Zdroj: | Journal of Chromatography A. 1032:73-77 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2004.01.013 |
| Popis: | Some biogenic amines, such as putrescine, cadaverine, spermidine and histamine, have been found to be useful as quality indices for the decomposition of fish, so research on the simultaneous analysis of various biogenic amines in food is of interest and importance. The intake of histamine may cause an allergic intoxication known as "scombroid poisoning" while secondary biogenic amines can potentiate the toxicity of histamine and in addition can react with nitrite to form carcinogenic nitrosamines. A new method for the simultaneous determination of underivatized biogenic amines based on ion-exchange chromatography with conductivity detector has been developed. The proposed method offers a number of advantages over previous pulsed amperometric detection and integrated pulsed amperometric detection methods such as simpler extraction procedure and sharp peaks. Separations were performed on a new low hydrophobic weak cation-exchange analytical column. This technique is simple, rapid and useful for routine checks in repetitive analyses. |
| Databáze: | OpenAIRE |
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