Characterization of a Sarcocystis neurona isolate from a Missouri horse with equine protozoal myeloencephalitis
| Autor: | José A. Ramos-Vara, Gayle C. Johnson, Philip J. Johnson, Antoinette E. Marsh |
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| Rok vydání: | 2001 |
| Předmět: |
Genetic Markers
Male Sarcocystosis Blotting Western Biology Equine protozoal myeloencephalitis Apicomplexa Antigen parasitic diseases Antigenic variation Animals Horses Internal transcribed spacer Encephalomyelitis General Veterinary Horse Sarcocystis General Medicine biology.organism_classification Virology Antigenic Variation Random Amplified Polymorphic DNA Technique Muscular Atrophy Phenotype Genetic marker biology.protein Parasitology DNA Intergenic Electrophoresis Polyacrylamide Gel Horse Diseases Antibody |
| Zdroj: | Veterinary parasitology. 95(2-4) |
| ISSN: | 0304-4017 |
| Popis: | Little information is available about antigenic variation of Sarcocystis neurona isolated from horses with equine protozoal myeloencephalitis, nor is there much information available on the specific antibody pattern to S. neurona antigens of horses from different geographic regions where S. neurona isolates have been obtained. This communication reports on the characterization of a new S. neurona isolate, SN-MU1. The isolate was obtained from a 3-year old Thoroughbred that had asymmetrical neurological signs and localized skeletal muscle atrophy. This S. neurona isolate is similar to other S. neurona isolates by molecular analysis of the internal transcribed spacer (ITS-1) region and a random-amplified polymorphic DNA marker, but is phenotypically distinct from the other S. neurona isolates examined. Evaluation of the antibodies from the affected horse and immunohistochemical results suggested that antigenic variation of S. neurona can result in variable antibody–antigen reactivity observed in the S. neurona immunoblot test. |
| Databáze: | OpenAIRE |
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