Flow cytometric characterization of microglia in the offspring of PolyI:C treated mice
| Autor: | Marie Pierre Manitz, Simone Wachholz, Astrid Friebe, Georg Juckel, Seray Demir, Jennifer Plümper, Manuela Eßlinger, Martin Eisenacher, Maike Ahrens |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Interferon Inducers Offspring Neuropathology Biology Flow cytometry Mice 03 medical and health sciences 0302 clinical medicine Pregnancy Calcium-binding protein medicine Animals Molecular Biology Sex Characteristics CD11b Antigen Interferon inducer medicine.diagnostic_test Microglia General Neuroscience Body Weight Calcium-Binding Proteins Microfilament Proteins Age Factors Gene Expression Regulation Developmental Flow Cytometry medicine.disease Poly I-C 030104 developmental biology medicine.anatomical_structure Animals Newborn Integrin alpha M Schizophrenia Prenatal Exposure Delayed Effects Immunology biology.protein Leukocyte Common Antigens Female Neurology (clinical) 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research. 1636:172-182 |
| ISSN: | 0006-8993 |
| Popis: | The neuropathology of schizophrenia has been reported to be closely associated with microglial activation. In a previous study, using the prenatal PolyI:C schizophrenia animal model, we showed an increase in cell numbers and a reduction in microglial branching in 30-day-old PolyI:C descendants, which suggests that there is microglial activation during adolescence. To provide more information about the activation state, we aimed to examine the expression levels of Iba1, which was reported to be up-regulated in activated microglia. We used a flow cytometric approach and investigated CD11b and CD45, two additional markers for the identification of microglial cells. We demonstrated that intracellular staining against Iba1 can be used as a reliable flow cytometric method for identification of microglial cells. Prenatal PolyI:C treatment had long-term effects on CD11b and CD45 expression. It also resulted in a trend towards increased Iba1 expression. Imbalance in CD11b, CD45, and Iba1 expression might contribute to impaired synaptic surveillance and enhanced activation/inflammatory activity of microglia in adult offspring. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect