Both excision and replication of cloned autonomous parvovirus DNA require the NS1 (rep) protein
Autor: | S L Rhode rd |
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Rok vydání: | 1989 |
Předmět: |
DNA Replication
Aphidicolin DNA polymerase Inverted repeat viruses Immunology DNA-Directed DNA Polymerase Viral Nonstructural Proteins Virus Replication medicine.disease_cause Microbiology Parvoviridae chemistry.chemical_compound Capsid Plasmid Virology medicine Humans Gene Mutation biology Viral Core Proteins DNA replication Molecular biology chemistry Viral replication Insect Science DNA Viral biology.protein Plasmids Research Article |
Zdroj: | Journal of Virology. 63:4249-4256 |
ISSN: | 1098-5514 0022-538X |
Popis: | When a bacterial plasmid containing the entire genome of LuIII virus except for the terminal 18 nucleotides from the right end is transfected into HeLa cells, the viral DNA is rescued and replicated, with production of infectious virus. This experimental system was used to examine the viral proteins and cis elements required for the excision and replication of viral DNA. The deletion of the entire NS1 gene provided a viral genome that was excised from the plasmid and replicated only when an NS1 gene was provided in trans. A frameshift mutation in the NS2 intron that truncates NS1 prevented excision and replication. Deletion of the left-end terminal inverted repeat or the right-end inverted repeat prevented excision of viral DNA from that end but not from the wild-type terminus. The viral terminus excised from the plasmid was protected from a processive degradation process, which began on the vector portion of the plasmid. The inhibitor of DNA polymerases alpha and delta, aphidicolin, blocked the excision reaction. |
Databáze: | OpenAIRE |
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