Response of the mouse sublingual gland to spaceflight
| Autor: | John Beallias, Arthur R. Hand, Didem Dagdeviren, Izaz Khan, M. I. Mednieks |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell type medicine.medical_specialty United States National Aeronautics and Space Administration Submandibular Gland Apoptosis Spaceflight Article law.invention Mice Sublingual Gland 03 medical and health sciences 0302 clinical medicine stomatognathic system law Internal medicine Autophagy medicine Animals Parotid Gland Salivary Proteins and Peptides Protein kinase A General Dentistry Cell Nucleus Weightlessness Chemistry Mucin Mucins 030206 dentistry Space Flight Serous demilune Cyclic AMP-Dependent Protein Kinases Immunohistochemistry United States Mice Inbred C57BL Serous fluid 030104 developmental biology Secretory protein Endocrinology Models Animal Astronauts Female |
| Zdroj: | European Journal of Oral Sciences. 126:373-381 |
| ISSN: | 1600-0722 0909-8836 |
| DOI: | 10.1111/eos.12541 |
| Popis: | The ultrastructure and immunohistochemistry of secretory proteins of sublingual glands were studied in mice flown on the US space shuttles Discovery [Space Transportation System (STS)-131] and Atlantis (STS-135). No differences in mucous acinar or serous demilune cell structure were observed between sublingual glands of ground (control) and flight mice. In contrast, previous studies showed autophagy and apoptosis of parotid serous acinar cells in flight mice. The expression of parotid secretory protein (PSP) in sublingual demilune cells of STS-131 flight mice was significantly increased compared with ground (control) mice but decreased in STS-135 flight mice. Similarly, expression of mucin (MUC-19) in acinar cells and expression of the type II regulatory subunit of protein kinase A (PKA-RII) in demilune cells were increased in STS-131 flight mice and decreased in STS-135 flight mice, but not significantly. Demilune cell and parotid protein (DCPP) was slightly decreased in mice from both flights, and nuclear PKA-RII was slightly increased. These results indicate that the response of salivary glands to spaceflight conditions varies among the different glands, cell types, and secretory proteins. Additionally, the spaceflight environment, including the effects of microgravity, modifies protein expression. Determining changes in salivary proteins may lead to development of non-invasive methods to assess the physiological status of astronauts. |
| Databáze: | OpenAIRE |
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