The Metagenome of Utricularia gibba's Traps: Into the Microbial Input to a Carnivorous Plant
| Autor: | Shamayim Martínez-Sánchez, Luis Herrera-Estrella, Enrique Ibarra-Laclette, Ignacio Torres, Luis David Alcaraz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lentibulariaceae lcsh:Medicine Plant Science Plant Genetics Genome Biochemistry Plant Genomics lcsh:Science Carnivorous plant Multidisciplinary biology Plant Bacterial Pathogens Microbiota Agriculture Genomics Plants Carnivory Lamiales Nucleic acids Ribosomal RNA Medical Microbiology Sequence Analysis Genome Plant Research Article Biotechnology Cell biology Cellular structures and organelles DNA Plant 030106 microbiology Plant Pathogens Crops Microbial Genomics Research and Analysis Methods Microbiology digestive system 03 medical and health sciences Model Organisms Plant and Algal Models Pseudomonas Botany Genetics Microbiome Grasses Non-coding RNA Molecular Biology Techniques Sequencing Techniques Molecular Biology Utricularia gibba Gene Library Carnivorous Plants Bacteria lcsh:R Organisms Biology and Life Sciences Plant Pathology biology.organism_classification 030104 developmental biology Metagenomics RNA Metagenome Plant Biotechnology lcsh:Q Rice Plant Structures Ribosomes Sequence Alignment Crop Science Cereal Crops |
| Zdroj: | PLoS ONE, Vol 11, Iss 2, p e0148979 (2016) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | The genome and transcriptome sequences of the aquatic, rootless, and carnivorous plant Utricularia gibba L. (Lentibulariaceae), were recently determined. Traps are necessary for U. gibba because they help the plant to survive in nutrient-deprived environments. The U. gibba's traps (Ugt) are specialized structures that have been proposed to selectively filter microbial inhabitants. To determine whether the traps indeed have a microbiome that differs, in composition or abundance, from the microbiome in the surrounding environment, we used whole-genome shotgun (WGS) metagenomics to describe both the taxonomic and functional diversity of the Ugt microbiome. We collected U. gibba plants from their natural habitat and directly sequenced the metagenome of the Ugt microbiome and its surrounding water. The total predicted number of species in the Ugt was more than 1,100. Using pan-genome fragment recruitment analysis, we were able to identify to the species level of some key Ugt players, such as Pseudomonas monteilii. Functional analysis of the Ugt metagenome suggests that the trap microbiome plays an important role in nutrient scavenging and assimilation while complementing the hydrolytic functions of the plant. |
| Databáze: | OpenAIRE |
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