Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants
| Autor: | Wei Li, Qingyun Diao, Fei Xue, Ting Zhou, Zhanbo Guo, Abebe Jenberie Wubie, Xiangfeng He, Shufa Xu |
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| Rok vydání: | 2014 |
| Předmět: |
Insecticides
Environmental Engineering Health Toxicology and Mutagenesis Mutant Microbiology Neonicotinoids chemistry.chemical_compound Plasmid Imidacloprid Restriction enzyme mediated integration Botany Environmental Chemistry Remi Trichoderma pBluescript biology Imidazoles Public Health Environmental and Occupational Health Neonicotinoid DNA Restriction Enzymes General Medicine General Chemistry Nitro Compounds biology.organism_classification Pollution Biodegradation Environmental chemistry Environmental Pollutants Genetic Engineering Plasmids |
| Zdroj: | Chemosphere. 112:526-530 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2014.01.023 |
| Popis: | REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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