Convergent evolution of cytochrome P450s underlies independent origins of keto-carotenoid pigmentation in animals
| Autor: | Yuki Manabe, Olivia Kosterlitz, Robert Greenhalgh, Nicky Wybouw, Richard M. Clark, John Vontas, Astrid Bryon, Thomas Van Leeuwen, Andre H. Kurlovs, Masahiro Osakabe |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
0106 biological sciences
Cytochrome Mutant Keto-carotenoids macromolecular substances Biology 01 natural sciences General Biochemistry Genetics and Molecular Biology Arthropod Proteins Evolution Molecular 03 medical and health sciences Cytochrome P-450 Enzyme System Convergent evolution biology.animal polycyclic compounds Animals Carotenoid Gene 030304 developmental biology General Environmental Science chemistry.chemical_classification Genetics 0303 health sciences General Immunology and Microbiology Phylogenetic tree Pigmentation organic chemicals Lemon Bulked segregant analysis Vertebrate food and beverages Biology and Life Sciences Genetics and Genomics General Medicine Carotenoid ketolase Carotenoids biological factors 010602 entomology chemistry biology.protein CYP384A1 General Agricultural and Biological Sciences Tetranychidae |
| Zdroj: | PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES Proceedings of the Royal Society B: Biological Sciences Proceedings of the Royal Society B: Biological Sciences 286 (2019) 1907 Proc Biol Sci Proceedings of the Royal Society B: Biological Sciences, 286(1907) |
| ISSN: | 1471-2954 0962-8452 |
| DOI: | 10.1098/rspb.2019.1039 |
| Popis: | Keto-carotenoids contribute to many important traits in animals, including vision and coloration. In a great number of animal species, keto-carotenoids are endogenously produced from carotenoids by carotenoid ketolases. Despite the ubiquity and functional importance of keto-carotenoids in animals, the underlying genetic architectures of their production have remained enigmatic. The body and eye colorations of spider mites (Arthropoda: Chelicerata) are determined by β-carotene and keto-carotenoid derivatives. Here, we focus on a carotenoid pigment mutant of the spider mite Tetranychus kanzawai that , as shown by chromatography, lost the ability to produce keto-carotenoids. We employed bulked segregant analysis and linked the causal locus to a single narrow genomic interval. The causal mutation was fine-mapped to a minimal candidate region that held only one complete gene, the cytochrome P450 monooxygenase CYP384A1 , of the CYP3 clan. Using a number of genomic approaches, we revealed that an inactivating deletion in the fourth exon of CYP384A1 caused the aberrant pigmentation. Phylogenetic analysis indicated that CYP384A1 is orthologous across mite species of the ancient Trombidiformes order where carotenoids typify eye and body coloration, suggesting a deeply conserved function of CYP384A1 as a carotenoid ketolase. Previously, CYP2J19, a cytochrome P450 of the CYP2 clan, has been identified as a carotenoid ketolase in birds and turtles. Our study shows that selection for endogenous production of keto-carotenoids led to convergent evolution, whereby cytochrome P450s were independently co-opted in vertebrate and invertebrate animal lineages. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|