| Autor: |
Aravinthan Loganathan, Fionnuala McMorrow, Hui Lu, Danyang Li, Ben Mulhearn, Neil John McHugh, Sarah Louise Tansley |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Loganathan, A, McMorrow, F, Lu, H, Li, D, Mulhearn, B, McHugh, N J & Tansley, S L 2022, ' The use of ELISA is comparable to immunoprecipitation in the detection of selected myositis-specific autoantibodies in a European population ', Frontiers in Immunology, vol. 13, 975939 . https://doi.org/10.3389/fimmu.2022.975939 |
| DOI: |
10.3389/fimmu.2022.975939 |
| Popis: |
BackgroundThe reliable detection of myositis-specific autoantibodies (MSA) provides valuable clinical information regarding prognosis, clinical progression and diagnostic confirmation.ObjectivesTo evaluate the reliability of a commercial ELISA immunoassay in detecting myositis-specific autoantibodies in comparison to immunoprecipitation as the reference standard.MethodsSerum samples were chosen from a biobank of more than 3000 samples. Samples with a confirmed MSA on Immunoprecipitation (n=116) were evaluated in duplicate by ELISA to detect Mi2, MDA5, Jo1, EJ, KS, PL-7 and PL-12 (Medical & Biological Laboratories Co. Ltd, Nagoya, Aichi, Japan). Healthy control samples (n=246) confirmed autoantibody negative by immunoprecipitation were similarly assessed.ResultsThere was a very good agreement between ELISA and immunoprecipitation for serum samples containing anti-Mi2, MDA5, Jo1, EJ, KS and PL-7 and PL-12 auto-antibodies. Cohen’s κ values ranged from 0.86-1 for the measured autoantibodies on ELISA.ConclusionELISA was an accurate method for detecting anti-synthetase, anti-Mi2 and anti-MDA5 autoantibodies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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