Mammalian oocytes store mRNAs in a mitochondria-associated membraneless compartment

Autor:	Shiya Cheng, Gerrit Altmeppen, Chun So, Luisa M. Welp, Sarah Penir, Torben Ruhwedel, Katerina Menelaou, Katarina Harasimov, Alexandra Stützer, Martyn Blayney, Kay Elder, Wiebke Möbius, Henning Urlaub, Melina Schuh
Rok vydání:	2022
Předmět:	Multidisciplinary Swine Egg Proteins Obstetrics and Gynecology RNA-Binding Proteins Hydrogels General Medicine Mitochondria RNA Messenger Stored Mice Oocytes Animals Humans Female Cattle
Zdroj:	Science (New York, N.Y.). 378(6617)
ISSN:	1095-9203
Popis:	Full-grown oocytes are transcriptionally silent and must stably maintain the messenger RNAs (mRNAs) needed for oocyte meiotic maturation and early embryonic development. However, where and how mammalian oocytes store maternal mRNAs is unclear. Here, we report that mammalian oocytes accumulate mRNAs in a mitochondria-associated ribonucleoprotein domain (MARDO). MARDO assembly around mitochondria was promoted by the RNA-binding protein ZAR1 and directed by an increase in mitochondrial membrane potential during oocyte growth. MARDO foci coalesced into hydrogel-like matrices that clustered mitochondria. Maternal mRNAs stored in the MARDO were translationally repressed. Loss of ZAR1 disrupted the MARDO, dispersed mitochondria, and caused a premature loss of MARDO-localized mRNAs. Thus, a mitochondria-associated membraneless compartment controls mitochondrial distribution and regulates maternal mRNA storage, translation, and decay to ensure fertility in mammals.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd1d4ab00c75ada0417f01262099afd8 https://pubmed.ncbi.nlm.nih.gov/36264786 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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