Experimental evidence supports the abscess theory of development of radicular cysts
| Autor: | P.N.R. Nair, Ulf Sjögren, Göran Sundqvist |
|---|---|
| Přispěvatelé: | University of Zurich, Nair, P N R |
| Rok vydání: | 2008 |
| Předmět: |
Keratinocytes
Pathology medicine.medical_specialty Time Factors Neutrophils Gingiva 610 Medicine & health Inflammatory cysts Basement Membrane Epithelium Rats Sprague-Dawley Necrosis Animal model Subcutaneous Tissue Microscopy Electron Transmission parasitic diseases Periodontal Abscess medicine Animals Abscess General Dentistry Radicular Cyst Fusobacterium nucleatum business.industry 3504 Oral Surgery Epithelial Cells medicine.disease 3500 General Dentistry 2746 Surgery Rats Sprague dawley 10182 Institute of Oral Biology stomatognathic diseases Disease Models Animal 2733 Otorhinolaryngology Otorhinolaryngology Connective Tissue Fusobacterium Infections Diffusion Chambers Culture Surgery Oral Surgery business |
| DOI: | 10.5167/uzh-9011 |
| Popis: | The objective of this study was to experimentally induce inflammatory cysts in an animal model so as to test the hypothesis that radicular cysts develop via the "abscess pathway."Twenty-eight perforated custom-made Teflon cages were surgically implanted into defined locations in the back of 7 Sprague Dawley rats. A week after the implantation of the cages, a known quantity of freshly grown, close allogeneic oral keratinocytes in phosphate buffer solution (PBS) was injected into each cage. One cage per animal was treated as the control that received only epithelial cells. The remaining 3 cages of each animal were trials. Seven days post epithelial cell inoculation; a suspension of 0.2 mL of Fusobacterium nucleatum (10(8) bacteria per mL) was injected into each of the 3 trial cages. Two, 12, and 24 weeks after the inoculation of the bacteria, the cages were taken out, and the tissue contents were fixed and processed by correlative light and transmission electron microscopy. Sixteen of the 21 trial cages could be processed and yielded results.Inoculations of epithelial cells followed 1 week later by F. nucleatum into tissue cages resulted in the development inflammatory cysts in 2 of the 16 cages. The 2 cages contained a total of 4 cystic sites. None of the control cages showed the presence of any cyst-like pathology.Inflammatory cysts were induced by initiating acute inflammatory foci (abscess/necrotic area) by bacterial injection that got enclosed by a proliferating epithelium. This finding provides strong experimental evidence in support of the "abscess theory" of development of radicular cysts. |
| Databáze: | OpenAIRE |
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