Localization of protein kinase C to UV-sensitive photoreceptors in the mouse retina
Autor: | B.E. Reese, D.L. Stull, P.T. Johnson, Kenneth C. Wikler, Emil Bogenmann |
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Rok vydání: | 1998 |
Předmět: |
Genetically modified mouse
Calbindins Opsin genetic structures Ultraviolet Rays Physiology Lipoproteins Population Immunocytochemistry Mice Transgenic Nerve Tissue Proteins Biology Mice S100 Calcium Binding Protein G Interneurons Bipolar neuron Hippocalcin Recoverin medicine Animals Eye Proteins Fluorescent Antibody Technique Indirect education Protein Kinase C Protein kinase C Genetics education.field_of_study Retina Reporter gene Calcium-Binding Proteins Rod Opsins Antibodies Monoclonal beta-Galactosidase eye diseases Sensory Systems Cell biology Mice Inbred C57BL medicine.anatomical_structure Lac Operon Retinal Cone Photoreceptor Cells sense organs |
Zdroj: | Visual Neuroscience. 15:87-95 |
ISSN: | 1469-8714 0952-5238 |
DOI: | 10.1017/s0952523898151155 |
Popis: | The present study has identified a population of cone photoreceptors in the murine retina that are uniquely immunoreactive for protein kinase C (PKC). Wavelength-sensitive cone subtypes are segregated along the dorso-ventral axis in the mouse retina with ventral retina occupied exclusively by ultraviolet wavelength-sensitive (UVWS) cones, and dorsal retina dominated by middle wavelength-sensitive cones. PKC-positive cones are found primarily in the ventral retina, and double-label immunocytochemistry using a short wavelength-sensitive opsin antibody confirms that they specifically correspond to the UVWS cone subtype. The PKC antibody, as documented in other mammals, also identifies rod bipolar cells in the mouse retina. UVWS cones and bipolar cells have previously been shown to share transcriptional regulatory elements, as observed in transgenic mice encoding a portion of the human SWS-opsin promoter controlling the lacZ reporter gene. In such mice, the transgene product, β-galactosidase, is expressed in populations of both cones and bipolar cells. The present study confirms that lacZ-expressing photoreceptors are indeed PKC-positive photoreceptors, but that the lacZ-expressing bipolar cells are not the PKC-positive rod bipolar cells. These cells must correspond to a type of cone bipolar cell. |
Databáze: | OpenAIRE |
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